نتایج جستجو برای: nucleic acid amplification techniques naat
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INTRODUCTION Genital Chlamydia trachomatis infection is the leading cause of bacterial sexually transmitted diseases in industrial countries, particularly among young people. The consequences of chlamydial infections may involve pelvic inflammatory disease, ectopic pregnancy and tubal factor infertility. METHODS Available tests for detection of chlamydia in men and women include culture in ti...
Sensitive diagnostic tests for infectious diseases often employ nucleic acid amplification technologies (NAATs). However, most NAAT assays, including many isothermal amplification methods, require power-dependent instrumentation for incubation. For use in low resource settings (LRS), diagnostics that do not require consistent electricity supply would be ideal. Recombinase polymerase amplificati...
Introduction: Transfusion of blood and its components is a life- saving manoeuvre but has life threatening hazards as well. Blood Service (BTS) covered by “Drug Cosmetics Act” legal implications. Hence, strict criteriae are followed while selecting donor, particularly emphasising on Transmissible Infections (TTI), which may improve safe transfusion practices. Aim: To study the seroprevalence Hu...
Abstract Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) ...
The selection of nucleic acid aptamers is an increasingly important approach to generate specific ligands binding to virtually any molecule of choice. However, selection-inherent amplification procedures are prone to artificial by-product formation that prohibits the enrichment of target-recognizing aptamers. Little is known about the formation of such by-products when employing nucleic acid li...
Fixed cells with different nucleic acid contents and scatter properties (low nucleic acid [LNA], high nucleic acid 1 [HNA1], and HNA2) were sorted by flow cytometry (FCM). For each sort, 10,000 cells were efficiently captured on poly-l-lysine-coated microplates, resulting in efficient and reproducible PCR amplification.
Since publication of CDC's 1993 guidelines (CDC, Recommendations for the prevention and management of Chlamydia trachomatis infections, 1993. MMWR 1993;42[No. RR-12]:1-39), nucleic acid amplification tests (NAATs) have been introduced as critical new tools to diagnose and treat C. trachomatis and Neisseria gonorrhoeae infections. NAATs for C. trachomatis are substantially more sensitive than pr...
This presentation will describe a recently developed SlipChip based microfluidic platform to perform digital nucleic acid amplification in a simple and low-cost format. Samples can be introduced into the SlipChip by pipetting without the use of complex instruments, and thousands of nanoliter volume compartments can be generated by simple slipping. A “yes-or-no” digital readout of end-point fluo...
Nucleic acid scanning by hybridization (NASBH) is a non-electrophoretic typing strategy that uses gridded oligonucleotides to reproducibly characterize arbitrarily amplified nucleic acid sequences. Membrane-bound arrays of terminally-degenerate oligonucleotides were hybridized to DNA amplification fingerprinting (DAF) products from enterohemorrhagic Escherichia coli O157:H7 isolates. Numerical ...
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