We describe an application of cDNA libraries constructed from total RNA to isolate simultaneously many differentially expressed cDNAs between a pair of near isogenic lines of mungbean in a short period of time. All ten selected individual cDNA clones, containing inserts ranging from 1.1–1.9 kb, clearly showed positive Northern blotting. Nucleotide sequencing indicated that six of the clones wer...

Objective. To determine the significance of high serum ferritin observed in Indigenous Australian patients on maintenance haemodialysis in the Northern Territory, we assessed the relationship between ferritin and transferrin saturation (TSAT) as measures of iron status and ferritin and C-reactive protein (CRP) as markers of inflammation. Methods. We performed a retrospective cohort analysis of ...

A simjan virus 40 genomic fragment containing the genes coding for the large T and small t antigens was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus downstream of the strong polyhedrin promoter. Infection of eucaryotic Spodoptera frugiperda (SF9) cells with this recombinant virus produced significant amounts of small t antigen and little or no la...

Among the main ethnic groups discussed in the survey are: (1) Dagbani Nanuni and Moore–Gurma of Northern Region; (2) Dagaare – WaliBirifor of Upper West Region; and (3) "Frafra" – Nankani, Talni, Bulsa, Nabit, Kusaal, and Grusi of Upper East Region. Northern Ghana comprises the three northernmost administrative regions of Ghana: the Upper West Region, Upper East Region and Nor...

System defects and policy-related exclusion in the development and utilization of energy resources bring the problem of rich poverty to energy and chemical industry base in Northern Shaanxi. The long existence of the rich poverty problem of energy and chemical industry base in Northern Shaanxi forms deep-seated constraints to the sustainable development of energy economy in Northern Shaanxi and...

Statistical methods for identifying differentially expressed genes from microarray data are evolving. We developed a test for the statistical significance of differential expression as a function of time. When applied to microarray data obtained from endothelial cells exposed to shearing for different durations, the new multi-group test (G-test) identified three times as many genes as the one-w...

Melioidosis is caused by the saprophytic organism Burkholderia pseudomallei. The use of the indirect hemagglutination assay (IHA) has found widespread use in areas endemic for this disease. Using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the Northern Territory of Australia. Based on a threshold titer of 1:40, the sensitivity of the IHA o...

Middle Point orbivirus (MPOV) was isolated in 1998 from a healthy cow pastured at Beatrice Hill farm, Middle Point (formerly Coastal Plains Research Station), 50 km east of Darwin in Australia's Northern Territory. The isolate could not be identified by using conventional serological tests, and electron microscopy indicated that it belongs to the family Reoviridae, genus Orbivirus. Genetic sequ...

The inducible expression of polyphenol oxidase (PPO), a presumed antiherbivore enzyme, was examined in hybrid poplar (Populus trichocarpa x Populus deltoides). Following mechanical wounding simulating insect damage, PPO activity increased dramatically in wounded and unwounded leaves on wounded plants beginning at 24 and 48 h, respectively. A hybrid poplar PPO cDNA was isolated and its nucleotid...

1. Introduction Beckman (1986) draws a distinction between stress-accent and non-stress-accent (i.e., pitch-accent) languages. Stress-accent languages, such as English, convey accentual prominence by a combination of phonetic cues, including pitch, intensity and duration. Non-stress-accent languages, on the other hand, use only pitch as a correlate of accent. Tokyo Japanese is an example of the...