نتایج جستجو برای: nitrocellulose paper hemagglutination inhibition hi

تعداد نتایج: 2065075  

2009
Iain Stephenson Alan Heath Diane Major Robert W. Newman Katja Hoschler Wang Junzi Jacqueline M. Katz Jerry P. Weir Maria C. Zambon John M. Wood

Hemagglutination-inhibition (HI) and neutralization are used to evaluate vaccines against influenza virus A (H5N1); however, poor standardization leads to interlaboratory variation of results. A candidate antibody standard (07/150) was prepared from pooled plasma of persons given clade 1 A/Vietnam/1194/2004 vaccine. To test human and sheep antiserum, 15 laboratories used HI and neutralization a...

2010
Mark I. Chen Ian G. Barr Gerald C. H. Koh Vernon J. Lee Caroline P. S. Lee Robert Shaw Cui Lin Jonathan Yap Alex R. Cook Boon Huan Tan Jin Phang Loh Timothy Barkham Vincent T. K. Chow Raymond T. P. Lin Yee-Sin Leo

BACKGROUND We describe the serological response following H1N1-2009 influenza A infections confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). METHODOLOGY AND PRINCIPAL FINDINGS The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample...

Journal: :Journal of medical virology 2000
J C de Jong W E Beyer A M Palache G F Rimmelzwaan A D Osterhaus

The success of influenza vaccination depends largely on the antigenic match between the influenza vaccine strains and the virus strains actually circulating during the season. In the past, this match has proved to be satisfactory in most seasons. In the 1997/1998 season, however, hemagglutination inhibition (HI) assays with ferret antisera indicated a considerable mismatch between the H3N2 vacc...

Journal: :Vaccine 2011
Phillip C Gauger Amy L Vincent Crystal L Loving Kelly M Lager Bruce H Janke Marcus E Kehrli James A Roth

Influenza is an economically important respiratory disease affecting swine world-wide with potential zoonotic implications. Genetic reassortment and drift has resulted in genetically and antigenically distinct swine influenza viruses (SIVs). Consequently, prevention of SIV infection is challenging due to the increased rate of genetic change and a potential lack of cross-protection between vacci...

2013
Joanne M. Langley Alfonso Carmona Martinez Archana Chatterjee Scott A. Halperin Shelly McNeil Keith S. Reisinger Naresh Aggarwal Li-Min Huang Ching-Tien Peng José Garcia-Sicilia Ignacio Salamanca de la Cueva Fernando Cabañas Consuelo Treviño-Garza Miguel Angel Rodríguez-Weber Manuel de la O Vijayalakshmi Chandrasekaran Walthère Dewé Aixue Liu Bruce L. Innis Varsha K. Jain

BACKGROUND Mismatch between circulating influenza B viruses (Yamagata and Victoria lineages) and vaccine strains occurs frequently. METHODS In a randomized controlled trial, immunogenicity and safety of an inactivated quadrivalent influenza vaccine candidate (QIV) versus trivalent inactivated influenza vaccine (TIV)-Victoria(Vic) and TIV-Yamagata(Yam) in children 3-17 years of age was evaluat...

Journal: :Journal of immunology 1998
K B Renegar G D Jackson J Mestecky

Secretory IgA (S-IgA), a major humoral mediator of mucosal immunity, is a polymeric Ig containing an unusual extra polypeptide, secretory component (SC), added during transcytosis through epithelial cells. Polymeric S-IgA is more effective than monomeric IgA (mIgA) and IgG in neutralizing viruses. It is not known whether this increased efficacy is due solely to the polymeric structure of the mo...

Journal: :Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 2004
Bradley C Long Tony L Goldberg Sabrina L Swenson Gene Erickson Gail Scherba

Hemagglutination inhibition (HI) has been a reliable method for determining porcine antibody levels to the well-characterized swine influenza virus (SIV) H1N1 and H3N2 subtypes. However, the recent emergence of the novel H1N2 serotype of SIV and the persistence of 2 other serotypes (H1N1 and H3N2) in the United States swine population represents a significant challenge to diagnostics. Both stan...

2008
Zoltan Vajo Lajos Kosa Istvan Szilvasy Zsuzsanna Pauliny Kálmán Bartha Ildiko Visontay Mate Jankovics Agnes Kis Istvan Jankovics

OBJECTIVE Seasonal vaccination has been consistently shown to significantly reduce morbidity and mortality because of influenza epidemics, even in healthy, working adults. Here we report the results of the yearly licensing studies of the past 11 influenza seasons (1997-2007) with a trivalent, inactivated whole virus vaccine with an aluminum phosphate adjuvant system. METHODS Sixty healthy vol...

Journal: :Kidney & blood pressure research 2016
Jaromír Eiselt Lukáš Kielberger Daniel Rajdl Jaroslav Racek Petr Pazdiora Lada Malánová

BACKGROUND/AIMS The immune response to influenza vaccine may be influenced by many factors, e.g. age, comorbidities or inflammation, and iron status. METHODS We studied the vaccine-induced production of hemagglutination-inhibition antibodies (HI) in 133 hemodialysis patients (HD) and 40 controls. To identify variables associated with the immune response, uni- and multivariate regression analy...

Journal: :Journal of clinical microbiology 1981
D B Budzko D F Jelinek B W Wilcke

Approximately 7% of the sera tested to determine the presence of rubella-specific antibodies by the hemagglutination inhibition test demonstrated abnormal patterns of reactivity, rendering the test unreadable. Another 3% of sera were shown to have false-positive titers as high as 1:128. When these abnormally reacting and false-positive sera were heated at 56 degrees C for 30 min after chemical ...

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