We examined the potential sources of error in automated cation-exchange chromatographic quantitation of plasma taurine, both in sample preparation and in the analysis. Principal sources of error include: use of serum instead of plasma, which produces gross overestimates; use of tripotassium ethylenediaminetetraacetate (EDTA) as anticoagulant in systems involving ninhydrin detection (a ninhydrin...

A new antibiotic, named laterosporamine, was isolated from the culture broth of Bacillus laterosporus 340-19. The antibiotic is active against Gram-positive and Gram-negative bacteria in vitro and in vivo. It is a water-soluble basic substance, positive to ninhydrin, SAKAGUCHI'S and DRAGENDORFF'S reagents. A non-peptidic structure with an approximate empirical formula C17H35N7O4 was suggested.

Free or eiisily extractable amino acids were determined by two-dimensional pnper chromatography in infarotcd and noninfarcted areas of the left ventricles of dog hearts after ligation of the anterior descending coronary artery. Changes in amino acid content in in fare ted areas were characterized by decreases in some of the major ninhydrin-reactive constituents and appeared coincidentally with ...

Interferon-beta-1a (IFN-β-1a) is used clinically in the treatment of multiple sclerosis. Similar to other biological molecules, IFN-β-1a has a relatively short serum half-life and is rapidly detected by the host’s immune system. PEGylation is a common approach to increase the blood circulation time of therapeutic proteins. In the present study, IFN-β-1a was PEGylated using linear methoxy polyet...

Amino groups were covalently introduced onto a polycaprolactone (PCL) surface by the reaction between 1,6-hexanediamine and the ester groups of PCL. The occurrence of the aminolysis and the introduction of free NH(2) groups were verified qualitatively by fluorescence spectroscopy, where rhodamine B isothiocyanate was employed to label NH(2) groups, and quantitatively by absorbance spectroscopy,...

A self-assembled supramolecular dimeric entity via an exceptionally short (2.404 Å) and strong (22.9 kcal mol(-1)) [O-H-O](-) hydrogen bond is the key to the special reactivity of vasarenes with fluoride salts. Vasarene is a self-assembled, vase-shaped compound, obtained by the reaction between ninhydrin and phloroglucinol. Analogous compounds are prepared by replacing the phloroglucinol with o...

Cellocidin is an antibacterial antibiotic discovered by SUZUKI et al.1) Although the antibiotic shows an excellent preventive effect against rice bacterial leaf blight, its use is restricted because of its phytotoxicity to rice plants. OKIMOTO and MISATO2) demonstrated cellocidin inhibited the metabolism of a-keto-glutarate to succinate in Xanthomonas oryzae. TANAKA et al.3) also found that cel...