The goal of these studies was to determine whether different calcium channel antagonists affect glucose transport in a neuronal cell line. Rat pheochromocytoma (PC-12) cells were treated with L-, T-, and N-type calcium channel antagonists before measurement of accumulation of 2-[(3)H]deoxyglucose (2-[(3)H]DG). The L-type channel antagonists nimodipine, nifedipine, verapamil, and diltiazem all i...

The goal of these studies was to determine if different calcium channel antagonists affect glucose transport in a neuronal cell line. For these studies, rat pheochromocytoma (PC12) cells were treated with L-, Tand N-type calcium channel antagonists prior to measuring the accumulation of H-2-deoxyglucose (H-2-DOG). The L-type channel antagonists, nimodipine, nifedipine, verapamil, and diltiazem ...

BACKGROUND AND PURPOSE Cerebral vasospasm remains a major problem in patients recovering from aneurysmal subarachnoid hemorrhage despite advances in medical, surgical, and endovascular care. Our purpose was to assess the efficacy of intra-arterial nimodipine, a calcium-channel blocker acting mainly on cerebral vessels, in preventing delayed neurologic deficits in patients with symptomatic vasos...

A rapid, simple and reproducible HPLC method was developed and validated for the analysis of nimodipine (NM) and/or its metabolite, oxidized nimodipine (OX–NM) in rat cerebrospinal fluid (CSF) and artificial CSF. The NM and OX–NM were eluted in less than 10 min with no interferences from the endogenous CSF peaks. Analysis was carried out on a Eurospher Performance (RP-C18, 250 × 4.6 mm) column ...

BACKGROUND AND PURPOSE Aging spontaneously hypertensive stroke-prone rats (SHR-SP) were previously shown to develop neocortical strokes. Because the hippocampal CA1 is selectively vulnerable to abnormal brain perfusion, the neuropathological effects of spontaneous strokes were investigated on specific neurochemical alterations in two major cell types of the hippocampal CA1 in SHR-SP. METHODS ...

It is known that digoxin, which is a liposoluble cardiac glycoside, is well absorbed from intestine. In the present study, the absorption rates of digoxin from rat duodenum and the proximal and terminal parts of small intestine were determined in vitro. The isolated everted duodenum and intestinal sacs were put into oxygenated Tyrode solution at 37 degrees C. The Tyrode solution on the outer, m...

We compared the effects of intravenous infusions of 40 micrograms/kg/min verapamil (n = 5), 0.5 microgram/kg/min nimodipine (n = 5), and 5 ng/kg/min prostacyclin (n = 6) and no treatment (n = 6) on local cerebral pH and local cerebral blood flow in middle cerebral artery-occluded rats 90 minutes after the ischemic insult. Local cerebral pH and local cerebral blood flow were determined simultane...

We studied acutely dissociated neurons from the dorsomedial (shell) region of the rat suprachiasmatic nucleus (SCN) with the aim of determining the ionic conductances that underlie spontaneous firing. Most isolated neurons were spontaneously active, firing rhythmically at an average frequency of 8 +/- 4 Hz. After application of TTX, oscillatory activity generally continued, but more slowly and ...

BACKGROUND AND PURPOSE The frequency and prognostic significance of neuroradiological findings after cardiac arrest are unknown. Using healthy volunteers as control subjects, we studied the magnetic resonance imaging (MRI) findings associated with cardiac arrest, adjusted for confounding factors. METHODS The presence of cerebral infarcts, leukoaraiosis, atrophy, and edema on ultra-low-field M...

Whether the calcium entry blocker, nimodipine, prevents the increase in the concentration of free fatty acids and metabolic disturbances during ischemia and promotes functional and metabolic recovery after recirculation were examined. Severe forebrain ischemia in rats was induced by four-vessel occlusion with mild hypotension. After 30 minutes of ischemia, recirculation was started by removal o...