Several studies highlight the role of inflammatory markers in thrombosis as well as in cancer. However, their combined role in cancer-associated deep vein thrombosis (DVT) and the molecular mechanisms, involved in its pathophysiology, needs further investigations. In the present study, C-reactive protein, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1β), matrix metal...

We investigated the involvement of NF-kB/Rel transcription factors that reportedly can inhibit apoptosis in various cell types in the antiapoptotic mechanism of the cytoprotectant amifostine. In the nontumorigenic murine myeloid progenitor 32D cells incubated with amifostine, we detected a reduction of the IkBa cytoplasmic levels by Western blotting and a raising of nuclear NF-kB/Rel complexes ...

All cancer cells require increased nutrient uptake to support proliferation. In this study, we investigated the signals that govern glucose uptake in B-cell lymphomas and determined that the inhibitor of NF-kB-kinase b (IKKb) induced glucose transporter-1 (GLUT1) membrane trafficking in both viral and spontaneous B-cell lymphomas. IKKb induced AKT activity, whereas IKKb-driven NF-kB transcripti...

BACKGROUND Despite over forty years of investigation on low-level light therapy (LLLT), the fundamental mechanisms underlying photobiomodulation at a cellular level remain unclear. METHODOLOGY/PRINCIPAL FINDINGS In this study, we isolated murine embryonic fibroblasts (MEF) from transgenic NF-kB luciferase reporter mice and studied their response to 810 nm laser radiation. Significant activati...

FIP-3 (14.7K interacting protein) was discovered during a search for cell proteins that could interact with an adenovirus protein (Ad E3–14.7K) that had been shown to prevent tumor necrosis factor (TNF)-a-induced cytolysis. FIP-3, which contains leucine zippers and a zinc finger domain, inhibits both basal and induced transcriptional activity of NF-kB and causes a late-appearing apoptosis with ...

NF-KB, purified from HeLa cell cytosol, and a recombinant p50 subunit of NF-KB alone (expressed in and purified from bacteria) both stimulated transcription from the HIV-1 promoter in vitro (at least up to 15-fold]. A deletion analysis of the p50 subunit revealed that transcriptional activation was mediated by the conserved c-re/-related domain. IKB-P (or a related protein), which binds to the ...

Purpose: To evaluate nuclear NF-kB translocation in minor salivary glands (mSG) of human primary Sjögren Syndrome (pSS). Methods: Lip biopsies’ mSG were done in 24 female patients with pSS from the Rheumatology Service of Rivadavia Hospital. Glands were stained with H&E and immunostained for NF-kB. Specimens were classified according to the Chisholm and Masson score. Results: The biopsies (H&E ...

Abstract BLOC1S1 is an adaptor protein that modulates endosome-lysosome function as a component of numerous multiprotein complexes. We postulated its modulation in CD4 +T cells may help us uncover the role biology adaptive immunity. In primary cre-recombinase knockout resulted preferential induction Th2 compared to Th1 and Th17 cytokine production following T cell receptor activation. As atopic...

IL-2 gene transcription is affected by several nuclear proteins. We asked whether dexamethasone (Dex) and cyclosporin A (CsA) inhibit IL-2 gene transcription by interfering with the activity of nuclear proteins that bind to the IL-2 promoter. Nuclear extracts from primary human T lymphocytes were analyzed by electrophoretic DNA mobility shift assays. Both Dex and CsA inhibited the binding of tr...