Newcastle disease causes many economic losses to the poultry industry in most countries. This disease is endemic in Iran. Backyard poultry is considered the reservoir of Newcastle virus; however, there is either no vaccination program against Newcastle, or it is performed in a restricted manner. Commercial live vaccines are inactive and sensitive to temperature; moreover, vaccine delivery to vi...

Newcastle Disease (ND) is an acute, mild to severe, highly infectious and pathogenic disease of domestic poultry, caged and aviary birds as well as wild birds caused by specified viruses of the Avian Paramyxovirus Type 1 (APMV-1) [1,2]. The disease is acute contagious which is characterized by sudden onset and rapid spread within the flock resulting high morbidity and mortality. Newcastle disea...

Newcastle disease virus (NDV) California strain reportedly grows poorly in L cells but replicates very well in chicken embryo cells. NDV-infected L cell cultures show a characteristic virus growth curve with respect to uridine incorporation, but plaque assays of the virus produced 24 h postinfection (PI) show no infectious particles when assayed on L cell monolayers and only a very low titer on...

We have generated GFP expressing recombinant Newcastle Disease Virus (rNDV-GFP) based on a live vaccine strain which contains a less pathogenic cleavage site of F protein leading to restricted viral growth in vivo. We evaluated the rNDV have an effect of oncolytic activity to B16 melanoma cell. The rNDV-GFP infected efficiency to B16 cells and induced cell death. Apoptosis-related genes, Bax an...

Newcastle Disease Virus (NDV), an avian paramyxovirus, is a highly contagious, generalised virus disease of domestic poultry and wild birds characterized by gastro-intestinal, respiratory and nervous signs. In this study, it was shown that NDV strain AF2240 and V4-UPM are cytolytic to Human Promyelocytic Leukemia, HL60 and Human T-lymphoblastic Leukemia, CEM-SS cells. Results from MTT cytolytic...

The co-transcriptional editing of the Newcastle disease virus (NDV) P gene has been studied by sequence analysis of cloned viral genomic RNA and mRNA. Evidence has been obtained for the specific insertion of non-templated G nucleotides, the consequence of which is the generation of three populations of P gene-derived mRNAs. The three populations encode proteins (P, V and W) which have a common ...

Circulating interferon production, induced by Newcastle disease virus, is about seven times higher in C57 Black mice than in Balb/c/Gif mice. A Mendelian analysis was carried out and circulating interferon production was measured in reciprocal F1 hybrids, in the F2 generation, in progeny of backcrosses of F1 hybrids to either parent strain, and in second backcross progeny. The results indicate ...