N-(rhodamine B)-deoxylactam-5-amino-1-pentanol (dRB-APOH) was designed and prepared as the chromo-fluorogenic sensor for detection of a nerve agent simulant via analyte triggered tandem phosphorylation and opening of the intramolecular deoxylactam. The successful detection of diethyl chlorophosphate suggests the utility of rhodamine-deoxylactams as the chromo-fluorogenic signal reporting platfo...

Sensor arrays based on industrial CMOS-technology combined with post-CMOS micromachining (CMOS MEMS) are a promising approach to low-cost sensors. In the first part of this article [1], the state of research on CMOS-based gas sensor systems was reviewed, and a platform technology for monolithic integration of three different transducers on a single chip was described. In this second part, the t...

We detect trace residues of RDX and TNT by surface photofragmentation fragment detection in real time at ambient conditions. An infrared or visible laser generates signature, NO molecular fragments, and a second, low energy, ultraviolet laser facilitates their detection by resonance enhanced multiphoton ionization. We present the effects of pump laser wavelength, laser energy, delay timing betw...

A dissolution method with robust high performance liquid chromatographic (HPLC) analysis for immediate release tablet formulation was developed and validated to meet the requirement as per International Conference on Harmonization (ICH) and United States Food and Drug Administration (USFDA) guidelines. The method involved the use of Agilent ZORBAX Eclipse XDB C18 column, and temperature was mai...

Strontium (Sr) is an element of toxicological concern due to its close chemical proximity Ca. In this work, Sr in sea salts collected from China and South Korea was analyzed by laser‐induced breakdown spectroscopy (LIBS). The precision could be improved using multiple filter‐paper sampling intensity normalization a weak Na I line as reference signal. analyte signal variation between pieces well...

BACKGROUND Biosensor-based detection provides a rapid and low-cost alternative to conventional analytical methods for revealing the presence of the contaminants in water as well as solid matrices. Although important to be detected, small analytes (few hundreds of Daltons) are an issue in biosensing since the signal they induce in the transducer, and specifically in a Quartz-Crystal Microbalance...

The analytical objective of developing sensitive, selective and rapid methods for the analysis of different substances in complex matrices has led to the production of several chemical sensors. Among the analytical applications of chemical sensors, the one involving the early detection of pathogen microorganisms can be considered essential in fields such as, • Food industry • Clinical and envir...

Under normal circumstances, a test specimen is reported as "negative" when the response of the analyte is absent. However, if the intensity of the internal standard (IS) is low, indicating interference factors, the test could be considered "inconclusive." A quantitative hypothesis, A=(R x I x S)/L, serves as the "cutoff" for the acceptable signal-to-noise (S/N) ratio for the IS in making "negat...

We developed a one-step real-time duplex reverse transcription PCR (RT-PCR) method using the LightCycler platform. This method allows simultaneous reverse transcription and real-time PCR amplification of two mRNAs of specific genes of interest (analyte genes) and mRNA of constantly transcribed genes (housekeeping genes) in a single-tube reaction. Specimen total nucleic acids were used because e...

A fiber optic bead-based sensor array platform has been employed to discriminate between six different odors and air carrier gas. Six different bead sensor types, with over 250 replicates of each, were monitored before, during, and after odor exposure to produce time-dependent fluorescence response patterns that were unique for each sensor-analyte combination. A total of 2,683 sensors were anal...