نتایج جستجو برای: nested polymerase chain reaction nested
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We performed serial nested reverse transcription polymerase chain reaction (RT-PCR) assays to monitor minimal residual disease (MRD) in 25 patients with t(8;21) acute myeloid leukemia (AML). We found transient disappearance of the AML1-ETO transcript in most of the patients in complete remission. Durable PCR-negativity was found in a subgroup of patients and was associated with a good prognosis.
INTRODUCTION Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) RNA in clinical samples have been extensively described. Routine commercial methods for each specific purpose (detection, quantification and genotyping) are also available, all of which are typically based on polymerase chain reaction (PCR) targeting the HCV 5' untranslated region (5'UTR). This st...
Analysis of specific gene expression in single living cells may become an important technique for cell biology. So far, no method has been available to detect mRNA in living cells without killing or destroying them. We have developed here a novel method to examine gene expression of living cells using an atomic force microscope (AFM). AFM tip was inserted into living cells to extract mRNAs. The...
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