objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...

ABSTRACT The period-change rate (PCR) of pulsating variable stars is a useful probe changes in their interior structure, and thus evolutionary stages. So far, the PCRs classical Cepheids Large Magellanic Cloud (LMC) have been explored limited sample total population these variables. Here, we use template-based method to build observed-minus-computed (O ? C) period diagrams, from which can deriv...

In recent years, more and people have begun to use massive online open course (MOOC) platforms for distance learning. However, due the space–time isolation between teachers students, negative emotional state of students in MOOC learning cannot be identified timely. Therefore, receive immediate feedback about their states. order identify classify learners’ emotions video scenarios, we propose a ...

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with ...

Genomic DNA preparations derived from mammalian cells can often exhibit poor template activity in PCR, particularly when carried out on target sequences present at low copy number. Using genomic DNA bearing SV40 sequences integrated into host chromosomal DNA at low copy number as a target, we show that template efficiency can be dramatically enhanced after treatment of the genomic template with...

We present a AHP based technique to grade and aggregate the processes and the projects in an organization. In particular, we present the application of group AHP to the problem of choosing process change requests (PCRs) in a software process improvement (SPI) program at IBM Global Services. In such situations, it is crucial to ensure that all PCRs are taken and evaluated in a transparent and co...

We used genus/species specific PCRs to determine the temporal persistence of host DNA in Triatoma infestans experimentally fed on blood from six common vertebrate species: humans, domestic dogs, guinea pigs, chickens, mice, and pigs. Twenty third or fourth instar nymphs per animal group were allowed to feed to engorgement, followed by fasting-maintenance in the insectary. At 7, 14, 21, or 28 da...