We evaluated a monoclonal fluorescent-antibody (FA) reagent (Neisseria gonorrhoeae Culture Confirmation Test; Syva Co., Palo Alto, Calif.) for confirmation of N. gonorrhoeae isolates obtained from clinics for sexually transmitted diseases in four cities. The FA test was performed in parallel with established confirmation procedures on all organisms growing on 773 primary culture plates of modif...

Hen antigonococcal lipopolysaccharide hen serum was used in a simple slide agglutination test for the identification of Neisseria gonorrhoeae from primary isolates.

Thirty-six strains of penicillinase-producing Neisseria gonorrhoeae (PPNG) were detected among 794 prostitutes between October 1981 and May 1982 in Surabaya, Indonesia. The high prevalence of PPNG strains among the high-class prostitutes and a lower prevalence among the low-class suggest that regular mass treatment for syphilis did not play an important role in the emergence and spread of PPNG;...

A concentration of 16 mg/l spectinomycin incorporated in agar gave the best discrimination between Neisseria gonorrhoeae sensitive and resistant to spectinomycin. This method was compared with spectinomycin sensitivity testing with 25 micrograms or 100 micrograms discs. Both methods agreed fully for 197 spectinomycin sensitive and three spectinomycin resistant gonococci. The agar incorporation ...

A modification of the rapid sugar fermentation procedure has been developed because of difficulties encountered in reproducing the reported results. Changes in the method have made the test more sensitive and reproducible.

During 35 months of selective in vitro cultivation, Neisseria gonorrhoeae cells retained their virulence for humans and were shown to be closely related to a particular colonial morphology. Saline-autoagglutinability was the only other characteristic distinguishing virulent from avirulent cells. Human responses to challenge with cells of the different colonial types were studied for their relat...

Pili, in addition to enhancing attachment of gonococci to tissue culture cells, appear to reduce association (attachment/ingestion) of Neisseria gonorrhoeae with mouse peritoneal macrophages in vitro.

The Aptima Combo 2 assay is proposed as a rapid method of diagnosing Neisseria gonorrhoeae endocarditis or other suspected disseminated gonococcal disease.

No difference in survival was observed when ultraviolet-irradiated gonococcal cells were subsequently incubated in the dark or exposed to photoreactivating light. This observation indicates that photoreactivation is absent in Neisseria gonorrhoeae.