The recruitment of leukocytes from blood is one the most important cellular processes in response to tissue damage and inflammation. This multi-step process includes rolling their adhesion endothelial cells (EC), culminating crossing EC barrier reach inflamed tissue. Galectin-8 galectin-9 expressed on immune system are part this can induce cell via binding oligolactosamine glycans. Similarly, t...

This heterogeneous assay for tobramycin involves fluorescein-labeled tobramycin, which competes with native unlabeled tobramycin for anti-tobramycin binding sites. Bound and free labeled antigen are separated by precipitation with a second antibody. Fluorescence intensity of the resuspended precipitate is inversely proportional to native tobramycin concentration. Background interference was con...

An enzyme L-lysine-A-oxidase has been obtained from Trichoderma harzianum Rifai F-180. The analysis of serum from CBA mice immunized five times with native L-lysine-A-oxidase intravenously, at a dose of 35 U/kg, showed that the preparation had virtually no effect on the immune response to Tdependent antigen. L-lysine-A-oxidase at a dose of 35 U/kg administered twice intradermally has no effect ...

Monoclonal antibodies against an alpha-helical region of the iron-containing, oxygen-binding protein myohemerythrin were isolated following immunization of mice with either the whole protein or a peptide homolog of the helix. Three distinct epitopes within the myohemerythrin helix were identified. The individual residues within two of these epitopes that were essential for antibody binding were...

BACKGROUND The generation of monoclonal antibodies specific for protein antigens usually depends on purified recombinant protein for both immunisation and hybridoma screening. Purification of recombinant protein in sufficient yield and purity is a tedious undertaking and can be demanding especially in the case of membrane proteins. Furthermore, antibodies generated against a purified recombinan...