A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. T...

A high-pressure liquid chromatographic method for the determination of norfloxacin or ciprofloxacin concentrations in body fluids was developed and compared with a standard bioassay. The high-pressure liquid chromatographic assay utilizes a reverse-phase C18 column, an internal standard, and fluorescence detection, with reproducibility studies yielding coefficients of variation ranging from 0.6...

A simple and rapid HPLC method for the determination of simvastatin using a C18-Hypersil column and acetonitril–phosphate buffer–methanol (5 : 3 : 1, v/v/v) as a mobile phase with detection at 230 nm was proposed. Commercial pharmaceutical tablets were analyzed with a linear range for simvastatin up to 1.884 mg % and a regression coefficient of 0.9995. The method is found to be precise, accurat...

Khuhawar et al. (1998) studied the HPLC determination of rifampicin, isoniazid and pyrazinamide in the pharmaceutical preparations. Isoniazid alone and along with pyrazinamide and rifampicin has been estimated by HPLC. Rifampicin and pyrazinamide were separated from the derivative of isoniazid and are simultaneously determined. The chromatography is carried out from C18 column. These drugs were...

The developed RP-HPLC depends on quantitative chromatographic separation of the studied drugs on a C18 column using phosphate Buffer:acetonitrile (60:40, v/v), pH=4.6 as a mobile phase delivered at constant flow rate of 1 mL.min-1 with UV detection at 210 nm. Calibration curves for Amlodipine besylate and Perindopril Arginine were constructed over the concentration range of 1-50 μg.mL-1 for bot...

We describe a rapid, reliable "high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0...

A new analytical method has been developed for the quantification of polybrominated diphenyl ethers (PBDEs) in human adipose tissue samples. After Soxhlet extraction and a cleanup procedure using two successive solid-phase extraction cartridges containing acid silica and acid silica: neutral silica:deactivated basic alumina (from top to bottom), detection can been achieved by narrow-bore (0.10-...

The feasibility of BOLD fMRI in association with electrical somatosensory stimulation on spontaneously breathing, isoflurane-anesthetized mice was investigated using spin-echo, echo-planar imaging (EPI) on a vertical narrow-bore 9.4 T magnet. Three experiments were performed to derive an optimal fMRI protocol. In Experiment 1 (n = 9), spin-echo BOLD responses to 10% CO2 challenge under graded i...

migrastatin isolate contained teleocidin-related compounds which display strong inhibitory activity against tumor cell migration. In this report, we describe the effects of teleocidin-free migrastatin on tumor cell migration and on the growth of several types of tumor cells. Whenthe original migrastatin sample was subjected to HPLC (Capcell Pak C18 column, 20X250mm) developing with 70% aq aceto...

Methods: Alpha tocopherol, gamma tocopherol, and retinol are assayed using fluorescence detection. Excitation/emission wavelengths are 295 nm/330 nm and 325 nm/470 nm for the analysis of both tocopherols and retinol, respectively. Retinol acetate is employed as the internal standard. The reversed phase method incorporates gradient elution with a mobile phase consisting of methanol and acetonitr...