Presystemic intestinal metabolism reduces the intestinal absorption and bioavailability of orally administered drugs. The factors affecting glucuronidation activity in Caco-2 cells seeded in Transwell (4.7 cm(2)) require clarification to establish an in-vitro system to assess intestinal glucuronidation metabolism for novel drug development. alpha-Naphthol (alpha-NA), a substrate for UGT1A6 in C...

We demonstrate the photocatalytic protonation of a silylenol ether using 7-bromo-2-naphthol as an ESPT catalyst with phenol as the sacrificial proton source. Greater than 95% conversion is achieved with 1 mol% catalyst. The reaction cycle is dependent on the significantly increased acidity of the catalyst in the excited state as well as the long lifetime for the triplet excited state of 7-bromo...

On November 28, 2009, as part of events marking the twenty-fifth anniversary of the disaster at the Union Carbide plant in Bhopal, gas survivors protested the contents of the report prepared by government scientists that mocked their complaints about contamination. The survivors shifted from the scientific document to a mediated lunch invitation performance, purporting to serve the same chemica...

The intestinal flora forms a complex ecosystem that metabolizes dietary and endogenous nutrients under primarily anaerobic conditions. The ingestion of azo dyes has been proposed as one source of potential genotoxic agents. Many intestinal bacteria are able to reduce the azo bond (termed azofission), which liberates the substituted naphthol compounds. The standard Ames test has not demonstrated...

The simultaneous determination of vitamin D2 and its isomers by high-speed liquid chromatography (HSLC) is described. Preferred operating conditions for the resolution and quantitation of vitamin D2 5,6-trans-vitamin D2, ergosterol, lumisterol2, isovitamin D2, isotachysterol2, tachysterol2, and previtamin D2 are as follows: column, "Zorbax" SIL (25 cm x 2.1 mm i.d.); pressure, 100-120 kg/cm2; t...

An indirect alkaline phosphatase immunoenzymatic staining method was developed to localise antigens in human embryo fibroblasts that have been induced by cytomegalovirus. The enzyme label was developed with a naphthol salt and fast blue to obtain a bright blue staining of the antigens that could be clearly visualised under an ordinary light microscope. The procedure is rapid, sensitive, and spe...

Arginine has been estimated by precipitation as the picrate and flavianate complexes, enzymically by the action of arginase followed by the estimation of the urea formed, microbiologically and colorimetrically (cf. Block & Boiling, 1947). The colorimetric method developed by Sakaguchi (1925a, b) has been most widely used. This method relies upon the formation of a red-coloured complex produced ...

a convenient and efficient procedure for the synthesis of 14-aryl or alkyl-14h-dibenzo[a,j]xanthenes by condensation of 2-naphthol and aldehydes in the presence of methylimidazolium hydrogensulfate, as a brönsted acidic ionic liquid ([hmim]hso4), is described. both aromatic and aliphatic aldehydes reactedeasily to afford the corresponding 14-aryl or alkyl-14h-dibenzo[a,j]xanthenes in good yield...