conclusions our experimental and in silico findings may provide new insight in the complex molecular pathways of drug resistance and also could assist design an efficient therapeutic strategy for aspergillosis treatment. results four out of five isolates revealed cyp51a and mdr1 mrna overexpression. interestingly, the isolate, which was negative for cyp51a and mdr1 mrna expression showed a high...

The antifungal susceptibilities of 79 oral Candida glabrata isolates to fluconazole and voriconazole were compared. The MICs at which 90% of the isolates tested were inhibited were 1 microg of voriconazole/ml and 32 microg of fluconazole/ml. Oral C. glabrata isolates for which the fluconazole MICs are elevated are commonly those for which the voriconazole MICs are elevated, but these increases ...

1. The uptake of liposomes containing the photoprotein obelin by rat isolated adipocytes was investigated with the aim of producing liposome-cell fusion, enabling obelin to be introduced into the cytoplasm of intact cells. 2. Incubation of liposomes containing obelin with rat isolated adipocytes resulted in a time-dependent uptake of entrapped obelin by the adipocytes. The uptake by adipocytes ...

Intracellular delivery of drugs and DNA is hampered by the cytotoxicity of some carrier systems (such as cationic lipids) and degradation of drugs or DNA as a results of endocytic processing. It has been shown that the coupling of HIV-1 TAT protein, or shorter TAT peptides derived from this protein, to other proteins and small colloidal particles facilitates their efficient intracellular delive...

the purpose of this study was to produce oil in water nano-emulsion by ultrasonication, and investigate the influence of inulin and β-cyclodextrin on the prosperities of nano-emulsion and morphology of spray dried microcapsules. the results showed that emulsion droplet size in samples containing β-cyclodextrin (8.28±0.11 nm) was less than inulin samples (97.12±4.2 nm). considering sem photos, β...

Inactive, lipid-depleted cytochrome oxidase was rapidly activated upon incubation with either neutral, basic, or acidic liposomes. When incubated with 0.5 to 10 times its weight in liposomes, the enzyme bound 20% of its weight in lipids. This amount of bound lipids probably corresponds to the “annular lipids” postulated to be tightly bound to cytochrome oxidase (Jest, P. C., Griffith, 0. H., Ca...

Voriconazole is a novel antifungal triazole that undergoes extensive oxidative metabolization involving several CYP450 isoenzymes. We report the case of a 14-year-old patient who received voriconazole concomitant with ciclosporin A as secondary antifungal prophylaxis after bone marrow transplantation. Temporary discontinuation of voriconazole due to worsening liver function tests (LFTs) resulte...

This observational study of the use of voriconazole conducted in Spain has identified reasons, characteristics, and forms of use of voriconazole in critically ill patients admitted to the ICU. Voriconale was used for directed treatment (63%), by the intravenous route (75%), as rescue treatment (41%) in severely ill patients (APACHE 21) with high need of resources and therapeutic interventions. ...

To the Editor—Despite increases in obesity, data to guide dosing of voriconazole in this population are lacking. We describe the dosing of intravenous voriconazole in an obese patient undergoing an allogeneic hematopoeitic cell transplant. A 30-year-old male underwent a matched unrelated donor allogeneic stem cell transplant (SCT) for the treatment of acute promyelocytic leukemia. On day 11 fol...

Mouse L929 cells were incubated with antibody-targeted liposomes containing oligodeoxyribonucleotides (oligomers). When the oligomer was a 15-mer complementary to the 5'-end region of the mRNA encoding the N protein of vesicular stomatitis virus, the cells became less permissive for multiplication of that virus; greater than 95% reduction of viral multiplication was achieved. Protection was not...