An important goal is to identify the direct activation domain (AD)-interacting components of the transcriptional machinery within the context of native complexes. Toward this end, we first demonstrate that the multisubunit TFIID, SAGA, mediator, and Swi/Snf coactivator complexes from transcriptionally competent whole-cell yeast extracts were all capable of specifically interacting with the prot...

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pat...

Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to ...

Intracellular delivery of functional macromolecules using peptide transduction domains (PTDs) is an exciting technology with both experimental and therapeutic applications. Recent data indicate that PTD-mediated transduction occurs via fluid-phase macropinocytosis involving an intracellular pH drop to approximately 5. Nitrilotriacetic acid (NTA)-coordinated metals avidly bind hexahistidine-tagg...

Patient 1 showed onset at the age of 28 with facial and limb dystonia, pyramidal signs, and cerebellar ataxia as well as bulging eyes and ophthalmoplegia. His father was 63 at disease onset and his mother 65. Moreover, their clinical manifestations were very mild compared with those of the sonnamely, mild cerebellar signs and mild to moderate peripheral nerve involvement without bulging eyes. C...

BACKGROUND Achalasia is a rare disease with a morbidity of 1 in 100,000, for which the exact mechanism of pathogenesis has not been clarified due to the small total number of patients. We herein report on our experience with two cases of familial achalasia in which the involvement of genetic inheritance was suspected. CASE PRESENTATION These cases consist of a man in his thirties and his moth...

The Laius complex is the father's complex of fear of the son as a potential rival in his own prerogatives. I t is considered more fundamental than the Freudian Oedipus complex which describes the son's rivalry with his father, founded on the son's sexual desire for his mother. Laius is the father of Oedipus. Graber3 holds that the original guilt rests with Laius (12, p. 17f.). Since Laius wante...

A 67-year-old married, unemployed, primary school-educated (5 years of education) female was born as one of identical twin sisters. The patient did not have any complaints when she was brought to our psychiatry clinic by her son in June 2013; however, her son reported that his mother believed that she could see her identical twin sister in the mirror, and that her identical twin was trapped in ...