Volume and type of medium, carbohydrate concentration, carbohydrate ratios, and inoculum level were investigated as possible factors influencing total colony counts of anaerobic rumen bacteria obtained in roll tubes (18 by 150 mm). Colony counts were lower when the rumen fluid was clarified by centrifugation before inclusion in the medium; however, decreasing the volume of 40% rumen fluid gluco...

Effects of different levels of cadmium (0.1, 1, 2, 4, 8, 16, 32, 64, 128, 256 mg/l of culture medium) were evaluated using gas production technique. In the glass vials used to measure gas production 30 mg of buffered rumen fluid (2:1 ratio of rumen fluid: buffer) poured in glass and cultured at 38.6 temperature. Volume and pressure of produced gas were measured with syringes and digital pressur...

Effects of different levels of cadmium (0.1, 1, 2, 4, 8, 16, 32, 64, 128, 256 mg/l of culture medium) were evaluated using gas production technique. In the glass vials used to measure gas production 30 mg of buffered rumen fluid (2:1 ratio of rumen fluid: buffer) poured in glass and cultured at 38.6 temperature. Volume and pressure of produced gas were measured with syringes and digital pressur...

In vitro gas production techniques simulate the rumen fermentation process and they have been used to evaluate the potential of feeds to supply nutrients to ruminants. Thus, effects of various levels of cobalt supplementation on gas production and rumen fermentation were investigated using an in vitro gas production technique. Ground samples (100 mg DM) of 70% wheat straw and 30% concentrate we...

The major aim of this study was to compare the morphology and activity of rumen protozoa of Khuzestan water buffalo and Holstein cow using in vitro digestibility and gas production parameters of steam treated sugarcane pith. Rumen fluid obtained from two buffalo and cow steers fed the same diet, 30:70 concentrate: forage. To separate rumen protozoa, antibiotic solution and fungicides were added...

introduction ensiling is less dependent to weather condition and farmers are trying to ensile forages for preserving them for livestock feeding. ensiling legumes because of their buffering capacity and high moisture content are difficult. also, ensiling alfalfa will lose up to %85 of its nitrogen content as non-protein nitrogen. adding some additives like carbohydrate sources will improve alfal...

Studies involving the consumption,metabolism, and elimination of terpenes by small ruminants consuming terpene-laden shrubs as well as those exploring the potential for natural products as rumen modifiers could benefit from a procedure that measures terpenes in both blood and rumen fluid and that is suitable for either serum or plasma. The objective of this study was to modify an existing proce...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...