background while tuberculosis (tb) can be diagnosed by microscopy and culture, the sensitivity of ziehl-neelsen staining is variable and culture results require 4 - 8 weeks to be determined. polymerase chain reaction (pcr) and its modifications, including nested pcr, might be promising methods for the rapid diagnosis of tb. objectives this study aimed to evaluate the performance of nested pcr o...

background one-third of the world’s population is infected with mycobacterium tuberculosis. investigation of toll-like receptors (tlrs) has revealed new information regarding the immunopathogenesis of this disease. toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. toll-like receptor 2 and tlr-4 have been identified in association with tuberculosis in...

conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...

HIGHLIGHTS A multiplex PCR to identifying mycobacteria of medical interest was evaluated. Mycobacterium tuberculosis can be differentiated from other with the assay. The assay showed high sensitivity and specificity in detecting M. avium complex.

Mycobacterium tuberculosis is the causative agent of the disease, tuberculosis and H37Rv is the most studied clinical strain. We use comparative genome analysis of Mycobacterium tuberculosis H37Rv and human for the identification of potential targets dataset. We used DEG (Database of Essential Genes) to identify essential genes in the H37Rv strain. The analysis shows that 628 of the 3989 genes ...

Tuberculosis remains a major public health problem in both developing and developed countries. Drug-resistant tuberculosis is an increasing health problem and serious challenge to tuberculosis (TB) control programs. Information about the susceptibility pattern of Mycobacterium tuberculosis isolates against anti-tuberculosis drugs is an important aspect to TB control. The objectives of the s...

In this work we present an automatic algorithm for detection of mycobacterium tuberculosis from stained sputum smear images applying a novel method of tubeness filtering in conjunction with Otsu thresholding and connected component analysis. This eliminates human effort and measurement error from the detection of the bacteria, which normally requires a lab technician or pathologist to load a gl...

We evaluated the performance of the DR. TBDR/NTM IVD kit, which was designed to detect Mycobacterium tuberculosis, rifampin-resistant M. tuberculosis, and nontuberculous mycobacteria, for detecting 110 positive and 50 negative cultures in Mycobacterium Growth Indicator Tubes. The accuracy rate of this kit for identification of Mycobacterium species was 95.5% (105/110).

Citation: Dang G, Cui Y, Wang L, Li T, Cui Z, Song N, Chen L, Pang H and Liu S (2018) Extracellular Sphingomyelinase Rv0888 of Mycobacterium tuberculosis Contributes to Pathological Lung Injury of Mycobacterium smegmatis in Mice via Inducing Formation of Neutrophil Extracellular Traps. Front. Immunol. 9:677. doi: 10.3389/fimmu.2018.00677 extracellular sphingomyelinase rv0888 of Mycobacterium tu...

A new, simple immunochromatographic assay for rapid identification of Mycobacterium tuberculosis complex in liquid cultures has been developed. The principle of the assay is binding of the Mycobacterium tuberculosis complex specific antigen to the monoclonal antibody conjugated on the test strip. The aim of this study is evaluation of the performance of immunochromatographic assay in identifica...