Vertnin (VRTN) variants have been associated with the number of thoracic vertebrae in European pigs, but the association has not been evidenced in Chinese indigenous pigs. In this study, we first performed a genome-wide association study in Chinese Erhualian pigs using one VRTN candidate causative mutation and the Illumina Porcine 60K SNP Beadchips. The VRTN mutation is significantly associated...

Rice breeding was conducted for a long time during historical times and is an important job in Vietnam because rice the major food domestic consumption export. In this review, we have provided comprehensive insight into importance of promising germplasm resources, achievements, approaches as well discussed challenges perspectives country. With wild relative resources with rich various genetic d...

This is the first description of the polymorphisms of arylalkylamine-N-acetyltransferase (AA-NAT) gene in Indian goats with different reproductive traits (twinning percentage and age of sexual maturity). Based on the important role of AA-NAT in reproduction, it is considered as a possible candidate gene for this trait. Two novel synonymous SNPs, C825T (exon2) and C1249T (exon3) were identified....

The Bulgarian orange-fruited mutant cultivar Oranzheva kapiya is characterized with a high content of the antioxidant β-carotene. To identify the mutation leading to the accumulation of β-carotene and develop a suitable molecular marker for this trait, the genes for geranyl-geranyl pyrophosphate synthase, capsanthin-capsorubin synthase and β-carotene hydroxylase were studied both in the mutant ...

Genomic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. We investigated mutations arising spontaneously in rice OsMsh6 mutants by specific-locus amplified fragment sequencing. Totally 994 single-nucleotide mutations were identified in three mutants and on average the mutation density is about 1/136.72 Kb per mutant line. T...

Thermo-sensitive genic male sterility (TGMS) is an important genetic means of two-line hybrid rice breeding. Pollen fertility in TGMS lines regulated by a single point mutation genes. Based on nucleotide polymorphisms (SNPs) and insertion-deletion mutations, Kompetitive Allele-Specific PCR (KASP) markers were developed utilized molecular breeding via high-throughput detection, which saves time ...

Food crop production and quality are two major attributes that ensure food security. Rice is one of the sources feeds half world’s population. Therefore, to feed about 10 billion people by 2050, there a need develop high-yielding grain rice varieties, with greater pace. Although conventional mutation breeding techniques have played significant role in development desired varieties past, due cer...

Electrophoretic screening of (C57BL/6J x DBA/2J)F1 progeny of male mice treated with N-ethyl-N-nitrosourea revealed a mouse that lacked the paternal carbonic anhydrase II (CA II). Breeding tests showed that this trait was heritable and due to a null mutation at the Car-2 locus on chromosome 3. Like humans with the same inherited enzyme defect, animals homozygous for the new null allele are runt...

Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the ...

Mutations of mtDNA are an important cause of human disease, but few animal models exist. Because mammalian mitochondria cannot be transfected, the development of mice with pathogenic mtDNA mutations has been challenging, and the main strategy has therefore been to introduce mutations found in cell lines into mouse embryos. Here, we describe a phenotype-driven strategy that is based on detecting...