Thymol and carvacrol are phenolic isomers with the potential developmental toxicity endocrine disruptions (ED) at low concentrations. However, few reports estimated their ED below 10−6 M (150 μg/L) (MW of thymol carvacrol: 150 g/mol). In this study, both chemicals were determined for 500 μg/kg 50 using chicken embryonic assay, estrogenic activity (EA) 10−12 to 10−7 (1.5 × 10−4 15 by MCF-7 cell ...

Introduction. The test for the induction of reverse gene mutations (Ames test, OECD* guideline No. 471) is one most popular methods assessing mutagenicity due to its ease execution and ability detect up 70-80% substances with carcinogenic activity. experimental protocol requires a minimum amount substance standard microbiological laboratory equipment. To obtain primary data, several days from s...

The objective of this study is to determine the antimutagenic effect honey and its correlation with concentration total polyphenolic content. Seven samples were collected from different regions Algeria. content was determined by Folin-Ciocalteu colorimetric assay. carried out AMES Salmonella/ microsome mutagenicity assay against three known mutagenic substances (4-nitro-o-phenylenediamine, sodi...

The Ames/Salmonella mutagenicity assay was used to screen processed oil shale extracts for potentially carcinogenic chemicals. Positive mutagenic activity was detected in organic solvent extracts of all four spent shales tested. This result combined with the fact that most carcinogens (90 percent) have been detected as mutagens in the Ames test, indicates that these extracts contain carcinogens...

The Salmonella lactam test is a newly developed method for detecting genotoxins. This technique is based on the ability of DNA damaging agents to reverse expression of the beta-lactamase gene, an important gene that enables microbes to resist beta-lactam antibiotics. A construct p-SELECT Control DNA plasmid containing a beta-lactamase gene site was constructed in many mutant forms, including po...