Abstract Crop genetic diversity has a significant role in improving new plants through breeding. The chrysanthemum contains the most mutant varieties, making mutation breeding one of widely utilized procedures for ornamental plants. goal this research is to use gamma irradiation induce variation and improve features. In vitro bud explants white ‘Bacardi’ type were treated with rays at 20 Gy on ...

Beyond the natural proteome, high-throughput mutagenesis offers the protein engineer an opportunity to "tweak" the wild-type activity of a protein to create a recombinant protein with required attributes. Of the various approaches available, saturation mutagenesis is one of the core techniques employed by protein engineers, and in recent times, nondegenerate saturation mutagenesis is emerging a...

In this work, we combined computational protein-protein docking with computational and experimental mutagenesis to predict the structure of the complex formed by monoclonal antibody 806 (mAb 806) and the epidermal growth factor receptor (EGFR). We docked mAb 806, an antitumor antibody, to its epitope of EGFR residues 287-302. Potential mAb 806-EGFR orientations were generated, and computational...