Background: Vascular endothelial growth factor-A (VEGF-A), an endothelial cell-specific mitogen produced by various cell types, plays important roles in cell differentiation and proliferation. In this study we investigated the effect of recombinant VEGF-A on differentiation of mesenchymal stem cells (MSCs) to endothelial cells (ECs). Methods: VEGF-A was expressed in E. coli BL21 (DE3) and BL21...

background: wharton’s jelly is an unlimited source of stem cells that can be used in cell therapy and tissue engineering without any ethical concern. it has been revealed the cell-free extract could be effective to induce cell differentiation. the objective of this study was to induce wharton’s jelly-derived mesenchymal stem cells (mscs) into hepatocyte-like cells by premeabilization of the cel...

multipotent mesenchymal stromal cells or mesenchymal stem cells (mscs) are mainly isolated from bone marrow or fat tissue. because of their potential of multilineage differentiation towards bone, cartilage and fat tissue, they were initially evaluated to develop innovative strategies for tissue engineering applications. more recently, they have gained interest based on their immunomodulatory pr...

The bone marrow serves as a reservoir for different classes of stem cells. In addtion to haematopoietic stem cells the bone marrow comprises a population of marrow stromal cells or mesenchymal stem cells (MSCs). Stromal cells exhibit multilineage differentiation capacity, and are able to generate progenitors with restricted developmental potential, including fibroblasts, osteoblasts, adipocytes...

umbilical cord blood (ucb) has been used for transplantation in the treatment of hematologic disorders as a source of hematopoietic stem cells (hscs). because of insufficient number of cord blood cd34+ cells, the expansion of these cells seems to be important for clinical application. mesenchymal stromal cells (mscs), playing an important role in hscs maintenance, were used as feeder layer. apo...

Mesenchymal stem cells (MSCs) are easily accessible and safe for regenerative medicine. MSCs exert trophic, immunomodulatory, anti-apoptotic, and tissue regeneration effects in a variety of tissues and organs, but their entity remains an enigma. Because MSCs are generally harvested from mesenchymal tissues, such as bone marrow, adipose tissue, or umbilical cord as adherent cells, MSCs comprise ...

purpose: to separate and characterize orbital fat-derived stem cells (ofscs) and compare its cellular and molecular properties with those of bone marrow-derived mesenchymal stem cells (mscs) and adipose-derived stem cells (ascs). methods: stem cells from orbital fat, abdominal fat and bone marrow aspirates have been isolated. we assessed stem cell specific cell surface markers using cytometry, ...

This study is the first documentation of the isolation and extensive characterization of mesenchymal stem cells from canine adipose tissue. Methods previously used by our group to isolate and differentiate human adipose-derived mesenchymal stem cells (hAD-MSCs) have been modified and optimized for derivation of similar cells from canine adipose tissues. The canine adipose tissue-derived mesench...

Numerous studies have been reported about the differentiation of mesenchymal stem cells (MSCs) into almost every cell type found in craniofacial tissues, such as bone, cartilage, tendon, ligament, muscle etc. Using MSCs as a cell source to engineer craniofacial tissue is becoming feasible. Some effects have been made to bridge the fundamental science and clinical application. Meanwhile, novel f...

Differentiation of derived from in vitro-incubated adult human mesenchymal stem cells (MSCs), isolated bone marrow, cartilage and adipose tissue, respectively, by application appropriate incubation conditions, to osteoclasts, was investigated proved. The current results show the importance changes mitochondrial dynamics about differentiation male germ (spermatogonia) mature functional activity ...