Avian bornaviruses were first described in 2008 as the causative agents of proventricular dilatation disease (PDD) parrots and their relatives (Psittaciformes). To date, 15 genetically highly diverse avian covering at least five viral species have been discovered different bird orders. Currently, primary diagnostic tool is detection RNA by conventional or real-time RT-PCR (rRT-PCR). One drawbac...

This study sought to establish a real-time reverse transcription (RT)-PCR method differentially detect canine distemper virus (CDV) wild-type and vaccine strains. To this end, pair of CDV universal primers two specific minor groove binder (MGB) probes, harboring T/C substitution in the hemagglutinin (H) gene, were designed. Using recombinant plasmid expressing H gene or strain as standards, sen...

INTRODUCTION This case report describes a rare condition: community-acquired adenovirus pneumonia in an immunocompetent adult. The diagnosis was achieved by using a multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and highlights the usefulness of these novel molecular diagnostic techniques in patients hospitalized with acute respiratory illness. We also perform...

A correction to this paper has been published: https://doi.org/10.1007/s10327-021-01008-7

Abstract Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries using DAS-ELISA or conventional real-time RT-qPCR. Previously, we developed a multiplex DiRT-PCR (Direct reverse transcript – polymerase chain reaction), which works directly on diluted tuber sap thus saves time chemical processing for RNA e...

A multiplex single-tube reverse transcription-polymerase chain reaction (RT-PCR) has been developed for the detection and differentiation of vesicular stomatitis viruses (VSV), Indiana 1 and New Jersey, from insect samples. Using this assay, detection of either or both viruses in as little as 20 fg of total RNA from tissue culture was achieved, along with detection of vesicular stomatitis (VS) ...