The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different species, including long non-coding RNAs (lncRNAs). Dense viral can multigenic transcripts in addition to commonly observed antisense transcripts. It is essential study the biological role...

OBJECTIVE: Apolipoprotein E (ApoE) is a glycoprotein that functions as ligand in receptor-mediated endocytosis of lipoprotein particles. There are studies showing the association APOE with neurogenerative diseases such Alzheimer's disease and multiple sclerosis (MS). The aim this study was to investigate whether there relationship between gene expression MS.MATERIAL AND METHODS: To determine ef...

The dromedary camels are essential because of their multiple uses for transport, wool, meat, and milk production. Camel’s is considered exceptional nutrition, so it therapeutic high nutrition. Mastitis may affect camel’s production, cause significant economic loss, associated with zoonotic disease. study aimed to detect the prevalence Mycoplasma spp. especially M. bovis in using polymerase chai...

BACKGROUND Preactivated solid surfaces provide new possibilities for multiple consecutive reactions in a microtiter plate format. In this study, a combination of PCR and subsequent hybridization in the same microtiter well was applied for the detection of HLA-B27 alleles. METHODS A multiplex solid-phase PCR to amplify the HLA-B27 alleles together with beta-actin as an amplification control ge...

Abstract Background Hypertrophy is a critical process for chondrocyte differentiation and maturation during endochondral ossification, which responsible the formation of long bone postnatal longitudinal growth. Increasing evidence suggests that melatonin, an indole hormone, plays pivotal role in chondrogenesis. However, little known about effects melatonin on terminal chondrocytes. Methods Mese...

A method based on amino-modified silica-coated magnetic nanoparticles (ASMNPs) and polymerase chain reaction (PCR) was developed to rapidly and sensitively detect foodborne pathogens in raw milk. After optimizing parameters such as pH, temperature, and time, a trace amount of genomic DNA of pathogens could be extracted directly from complex matrices such as raw milk using ASMNPs. The magnetical...

BACKGROUND Chlamydophila pneumoniae has been postulated as an aetiological agent in the pathophysiology of multiple sclerosis. Previous studies show conflicting results. OBJECTIVE To investigate patients with multiple sclerosis and other neurological diseases for evidence of past or present infection with C pneumoniae. METHODS 19 patients with multiple sclerosis and 29 with other neurologic...

In this paper we report that the inclusion of heat-resistant RecA protein from a thermophilic bacteria, Thermus thermophilus, and its cofactor (ATP) in PCR effectively eliminates non-specific PCR products. The effect of RecA protein, which catalyzes pairing between homologous DNA molecules with great fidelity in genetic recombination, is due to its promotion of precise priming in PCR (i.e. prim...