Proteins possess a number of attractive properties that have contributed to their recent emergence as nanoscale building blocks for biomaterials and bioinspired materials. For instance, the amino acid sequence of a protein can be precisely controlled and manipulated via recombinant DNA technology, and proteins can be biosynthesized with very high purity and virtually perfect monodispersity. Mos...

The widespread potential application of vertically aligned carbon nanotube (CNT) forests have stimulated recent work on large-area chemical vapor deposition growth methods, but improved control of the catalyst particles is needed to overcome limitations to the monodispersity and packing density of the CNTs. In particular, traditional thin-film deposition methods are not ideal due to their vacuu...

Fluorophore-labeled glycodendrimers have potential use in the study of carbohydrate-protein interactions by fluorescence spectroscopy and imaging methods. The current solution-phase methods for preparation of such glycoconjugates are labour intensive. On the other hand, the intrinsically more efficient solid-phase methods have been explored only at low generations. Herein we disclose a direct, ...

Multiplexed electrospray is a promising aerosol technique to achieve both high throughput and unique advantages of the electrospray such as monodispersity and small droplet size down to nanometer range. Here we study the interaction and overall deposition pattern of multiplexed electrosprays. First, we derived an approximate model for the profile of a single electrospray subjected to its own sp...

BACKGROUND Recent successes in the determination of G-protein coupled receptor (GPCR) structures have relied on the ability of receptor variants to overcome difficulties in expression and purification. Therefore, the quick screening of functionally expressed stable receptor variants is vital. RESULTS We developed a platform using Saccharomyces cerevisiae for the rapid construction and evaluat...

Membrane transporter proteins play critical physiological roles in the cell and constitute 5-10% of prokaryotic and eukaryotic genomes. High-resolution structural information is essential for understanding the functional mechanism of these proteins. A prerequisite for structural study is to overexpress such proteins in large quantities. In the last few years, over 20 bacterial membrane transpor...

A novel photo-crosslinkable nanogel is prepared from a biodegradable polymer template with intrinsic photoluminescence and high photostability. The fluorescent nanogels display excellent biodegradability and cytocompatibility owed to the facile synthesis scheme involving a solvent-and surfactant-free one-pot reaction, derived entirely from biocompatible monomers citric acid, maleic acid, L-cyst...

Pim kinases, including Pim-1, Pim-2 and Pim-3, belong to a distinctive serine/threonine protein-kinase family. They are involved in cytokine-induced signal transduction and the development of lymphoid malignancies. Their kinase domains are highly homologous to one another, but share low sequence identity to other kinases. Specifically, there are two proline residues in the conserved hinge-regio...

Both the diversity and complexity of microfluidic systems have experienced a tremendous progress over last decades, enabled by new materials, novel device concepts innovative fabrication routes. In particular subfield high-throughput screening, used for biochemical, genetic pharmacological samples, has extensively emerged from developments in droplet microfluidics. More recently, 3D architectur...

Monodisperse spherical silica particles, as a basis for creating supramolecular 3D matrices, are currently of increasing interest due to the prospects their wide application in synthesis new nanocomposite materials. At same time, one main problems large-scale is associated with unstable behavior tetraethoxysilane (TEOS) during hydrolysis, which leads both deviation particles from given size and...