Trapping quaternary structures of hemoglobin in single crystals or by encapsulation in silica gels has provided a demanding set of data to test statistical mechanical models of allostery. In this work, we compare the results of those experiments with predictions of the four major allosteric models for hemoglobin: the quaternary two-state model of Monod, Wyman, and Changeux; the tertiary two-sta...

To understand why the classical two-state allosteric model of Monod, Wyman, and Changeux explains cooperative oxygen binding by hemoglobin but does not explain changes in oxygen affinity by allosteric inhibitors, we have investigated the kinetic properties of unstable conformations transiently trapped by encapsulation in silica gels. Conformational trapping reveals that after nanosecond photodi...

Between 1957 and 1959, Arthur Pardee, François Jacob, and Jacques Monod [5] conducted a set of experiments at the Pasteur Institute in Paris, France, that was later called the PaJaMa Experiments, a moniker derived from the researchers' last names. In these experiments, they described how genes [6] of a species of single-celled bacteria, called Escherichia coli [7] (E. coli ), controlled the pro...

The discovery of regulator and operator genes, summarized and organized into a model for repressive regulation by Jacob and Monod 1r1 in il6t, defined a fundamental logical circuitry for control of gene expreision. thai celebrated formulation is surely one of the great achievements of the first century of the Institut Pasteur. chemical entities that execute this logic, the repressors and operat...

In this paper the problem of parameter identification for the Monod model is considered. As known for a long time, noisy batch measurements do not allow unique and accurate estimation of the kinetic parameters of the Monod model. Techniques of optimal experiment design are, therefore, addressed to design informative experiments and improve the parameter estimation accuracy. During the design pr...