Next-generation sequencing (NGS) technologies have enabled genome re-sequencing for exploring genome-wide polymorphisms among individuals, as well as targeted re-sequencing for the rapid and simultaneous detection of polymorphisms in genes associated with various biological functions. Therefore, a simple and robust method for targeted re-sequencing should facilitate genotyping in a wide range o...

• Histological examination of a tissue biopsy is required to differentiate between different lung cancer histiotypes. • In non-small-cell lung cancer, judicious use of a limited immunohistochemistry panel is recommended to differentiate between squamous cell and adenocarcinoma, and maximize tumor material for molecular analyses. • Clinical selection criteria should not be used to select patient...

The application of CRISPR/Cas9 technology has revolutionised genetics by greatly enhancing the efficacy of genome editing in the early embryo. Furthermore, the system has enabled the generation of allele types previously incompatible with in vivo mutagenesis. Despite its versatility and ease of implementation, CRISPR/Cas9 editing outcome is unpredictable and can generate mosaic founders. Theref...

The morphological and molecular diversity of 101 peanut varieties from South China were analyzed to identify distinctness among these varieties. No significant difference was observed for six characteristics whereas a range 0.25–0.51 index 11 characteristics, with an average value 0.39. Molecular characterization 40 highly polymorphic simple sequence repeats (SSRs) generated total 167 alleles r...

objective(s): candidiasis infection caused by candida albicans has been known as a major problem in patients with immune disorders. the objective of this study was to genotype the c. albicans isolates obtained from oral cavity of patients with positive human immunodeficiency virus (hiv+) with or/and without oropharyngeal candidiasis (opc). materials and methods:a total of 100 c. albicans isolat...

background: the present study was aimed to investigate molecular diversity of echino-coccus granulosus isolates collected from human clinical samples using two mitochondrial genes cox1 and nad1 in iran . methods: forty seven human hydatid cysts were collected through surgery from two hospitals in tehran during 2010-2012. to determine the fertility of protoscoleces, the cyst fluids were subjecte...

background: hydatidosis or cystic hydatid disease is one of the most important diseases in human and animals. identification of strains is important for improvement of control and prevention of dis-ease. the aim of this study was to determine the strains isolated from human and domestic animals in ilam province, iran, using pcr-rflp method. methods: respectively, 30 and 4 animal and human hydat...

Breeding by design has been an aspiration of researchers in the plant sciences for a decade. With the rapid development of genomics-based genotyping platforms and available of hundreds of functional genes/alleles in related to important traits, however, it may now be possible to turn this enduring ambition into a practical reality. Rice has a relatively simple genome comparing to other crops, a...

The avian and human pathogen Chlamydophila (C.) psittaci represents a genetically heterogeneous species. To facilitate epidemiological surveys, more rapid yet highly specific molecular tests are needed. Currently used typing methods, i.e. serotyping and PCR-RFLP, have only limited sensitivity and are incapable of covering the wide spectrum of naturally occurring types of C. psittaci strains. In...

context: detection of causative pathogen is crucial for treatment strategies in most infections. molecular diagnosis as a more sensitive and specific method, has become the best option to investigate certain pathogens, mainly viruses. recent scientific and technological approaches resulted in changing the area of diagnostic microbiology. evidence acquisition: a comprehensive online search of da...