A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-associated turbidity or primary amine content. The assay was shown to be sensitive to as little as 0.3 p g of lipoprotein and to yield similar results ...

Objective(s):EMAP-like Protein 5 (EML5) is a new echinoderm microtubule-associated protein that is expressed in the central nervous system. The aim of this study was to investigate the expression profile of EML5 in the anterior temporal neocortex of patients presenting with intractable epilepsy (IE). Materials and Methods:Western blot assays were performed to determine EML5 expression in 36 su...

Lysozyme, as a model protein, was precipitated through the formation of protein-Zn complex to micronize for subsequent encapsulation within poly (lactic-co-glycolic acid) (PLGA) microspheres. Various parameters, including pH, type and concentration of added salts and protein concentration, were modified to optimize the yield of protein complexation and precipitation. The resulting protein parti...

A departure from a radioenzymic procedure widely used for plasma catecholamine assay is reported, in which we use "mean net standards" instead of individual internal standards. Interlaboratory/intermethod analyses validated use of the modified principle and demonstrated its preferential use over the conventional principle. Also demonstrated is use of the modified procedure for calculating catec...

When total protein in very low density lipoprotein samples is measured by the method of Lowry et al. (1951. J. Biol. Chem 193:265-275), turbidity remains in the final color reaction. Addition of 0.1 ml of 2.5% (v/v) solution of Triton X-100 removes this turbidity immediately and effectively. This simple modification is faster, less cumbersome, and more economical than removing turbidity with et...

diminishing protein aggregation by chaperone is very important factor in medicine and industry. in this paper, itis induced the chaperone ability for 0-casein upon modification of its acidic residues by woodward reagentk(wrk) and examined on lysozyme as a target protein at ph 7.2 and outlined the mechanism for chaperoneability of modified system by uv-vis and fluorescence spectroscopy and theor...

Kathryn Calame C. Thomas Caskey Dennis W. Choi John M. Coffin Paul J. Crutzen Robert Desimone Nicole Le Douarin Bruce F. Eldridge Paul T. Englund Richard G. Fairbanks Douglas T. Fearon Harry A. Fozzard K. Friedrich Theodore H. Geballe Margaret J. Geller John C. Gerhart Roger 1. M. Glass Stephen P. Goff Peter N. Goodfellow Corey S. Goodman Stephen J. Gould ira Herskowitz Eric F. Johnson Stephen ...

The inhibitors may potentially be used for multiple therapeutic application in viral, bacterial, fungal disease and physiological disorder. In traditional Indian medicine system cassia tora is reportedly effective in treatment of skin and gastrointestinal disorder. The present work describe the isolation extraction purification characterization of trypsin inhibitor from cassia tora seeds. Purif...

A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-associated turbidity or primary amine content. The assay was shown to be sensitive to as little as 0.3 p g of lipoprotein and to yield similar results ...

Protein-protein interactions contribute to a variety of biological processes, including signal transduction, tissue integrity, and force generation (1,2). An inexpensive and reliable method to measure protein-protein interactions would be of considerable utility. Currently, co-immunoprecipitations (Co-IP) are routinely used to detect protein-protein interactions in cells, where one antibody is ...