MicroRNAs (miRNAs) encapsulated in extracellular vesicles (EVs) are potential diagnostic and prognostic biomarkers. However, discrepancies miRNA patterns their validation still frequent due to differences sample origin, EV isolation, sequencing methods. The aim of the present study is find a reliable isolation method for sequencing, adequate clinical application. To this aim, two comparative st...

Abstract Objectives This study aimed to examine the role of miR-221, miR-650, and miR-4534 expressions in development, pathogenesis, early diagnosis prostate cancer. Methods The included 83 participants: 37 patients with PCa, 31 BPH, 15 healthy subjects. MiRNA plasma samples was evaluated by quantitative RT-PCR. Results Plasma miR-221 levels were significantly upregulated PCa BPH groups compare...

MicroRNAs (miRNAs) are small preserved non-coding RNA molecules that regulate gene expression post-transcriptionally by targeting the 3' UTR of mRNAs for translational repression or degradation. The rising evidence has established the significant role of miRNAs within the regulation of immune system and the prevention of autoimmunity. MiR-146a has obtained an importance as a modulator of differ...

INTRODUCTION MicroRNAs (miRNAs), endogenous small noncoding RNAs regulating the activities of target mRNAs and cellular processes, are present in human plasma in a stable form. In this study, we investigated whether miRNAs are also stably present in synovial fluids and whether plasma and synovial fluid miRNAs could be biomarkers of rheumatoid arthritis (RA) and osteoarthritis (OA). METHODS We...

Blood feeding is an integral process required for physiological functions and propagation of the malaria vector Anopheles. During blood feeding, presence of the malaria parasite, Plasmodium in the blood induces several host effector molecules including microRNAs which play important roles in the development and maturation of the parasite within the mosquito. The present study was undertaken to ...