MicroRNAs (miRNAs) are 21-22 nucleotide regulatory small RNAs that repress message translation via base-pairing with complementary sequences in the 3' untranslated region (3'UTR) of targeted transcripts. To date, it is still difficult to find a true miRNA target due to lack of a clear understanding of how miRNAs functionally interact with their targeted transcripts for efficient repression. Pre...

Animal miRNAs are a large class of small regulatory RNAs that are known to directly and negatively regulate the expression of a large fraction of all protein encoding genes. The identification and characterization of miRNA targets is thus a fundamental problem in biology. miRNAs regulate target genes by binding to 3' untranslated regions (3'UTRs) of target mRNAs, and multiple binding sites for ...

Introduction. The development of new approaches to screening colorectal cancer (CRC), including those based on such molecular genetic methods as the assessment microRNA(miRNA) expression, is an urgent clinical task. Purpose study. study was aimed at a model for CRC prediction miRNA-21 expression level in saliva and plasma. Materials Methods. levels plasma (P-miRNA-21) saliva(S-miRNA-21) samples...

MicroRNAs (miRNAs) are ,22-nt regulatory RNAs expressed by all multicellular eukaryotes [1]. Humans encode .700 miRNAs and similar numbers are likely to exist in other mammalian species. Almost all cellular miRNAs are initially transcribed by RNA polymerase II (Pol II) as part of a long, capped, polyadenylated primary miRNA (pri-miRNA) precursor. The miRNA forms part of one arm of an RNA stem-l...

Animal microRNAs (miRNAs) guide proteins to repress the translation of target mRNAs via imperfect base pairing between the miRNA and the target. Computational analyses suggest that each miRNA regulates tens or hundreds of targets [1, 2], yet genetic studies usually show that the repression of a few targets plays a physiological role [3-5]. The extent of miRNA-mediated repression (which rarely e...

Expression of miRNAs in Neuroendocrine Neoplasms (NEN) is poorly characterized. We therefore wanted to examine the miRNA expression in Neuroendocrine Tumors (NETs), and identify their targets and importance in NET carcinogenesis. miRNA expression in six NEN primary tumors, six NEN metastases and four normal intestinal tissues was characterized using miRNA arrays, and validated by in-situ hybrid...

MicroRNAs (miRNA) are small RNA molecules of approximately 20 to 22 nucleotides that reduce expression of proteins through mRNA degradation and/or translational silencing. Each known miRNA has a large number of predicted targets. Members of the let-7/miR-98 family of miRNAs are up-regulated at the end of embryonic development. Let-7 is often down-regulated early during cancer development, sugge...

Most follicles in the mammalian ovary undergo atresia. Granulosa cell apoptosis is a hallmark of follicle atresia. Our previous study using a microRNA (miRNA) microarray showed that the let-7 microRNA family was differentially expressed during follicular atresia. However, whether the let-7 miRNA family members are related to porcine (Sus scrofa) ovary follicular apoptosis is unclear. In the cur...

Ginkgo biloba leaves contain abundant flavonoids, and flavonoid accumulation is affected by age. MicroRNAs (miRNAs) play an important role in the plant aging pathway. However, miRNAs involved biosynthesis related to age G. have rarely been studied. In this study, we compared 1-, 4-, 7-year-old ginkgo seedings found a significant decrease content of quercetin, kaempferol, total flavonol aglycone...