A BASIC program is presented which facilitates the formulation of biologically relevant chemical solutions containing specified free concentrations of as many as three divalent metal cations (Ca2+, Mg2+ and the choice of a third divalent cation) at any pH in the presence of as many as three ligands (EGTA, ATP and GTP). The program uses the law of mass action and the absolute stability (associat...

The extent of glucose and fructose utilization during cucumber juice fermentation was affected differentially by the addition of 10 to 360 mM of selected anions (chloride, nitrate, sulfate, phosphate, acetate, lactate, and citrate) and cations (Na+, K+, NH 4 +, Ca2+, Mg2+, and Mn2+). Inorganic anions generally suppressed utilization of both sugars, particularly at higher concentrations. Acetate...

The influence of the chemical composition natural waters on triclocarban (TCC) sorption pristine and irradiated multi-walled carbon nanotubes (MWCNTs) at different temperatures was studied. Natural have been characterized in terms concentrations cations anions, pH, electric conductivity. process TCC MWCNTs is influenced by both variation temperature. adsorption capacity studied increased increa...

Microbial fossils preserved by early diagenetic chert provide a window into the Proterozoic biosphere, but seawater chemistry, microbial processes, and interactions between microbes environment that contributed to this preservation are not well constrained. Here, we use fossilization experiments explore processes preserve marine cyanobacterial biofilms precipitation of amorphous silica in mediu...

A nucleoside triphosphatase (NTPase) present in highly purified preparations of pea nuclei was partially characterized. The activity of this enzyme was stimulated by divalent cations (Mg2+ = Mn2+ > Ca2+), but was not affected by the monovalent cations, Na+ and K+. The Mg(2+)-dependent activity was further stimulated by concentrations of Ca2+ in the low micromolar range. It could catalyze the...

The divalent cations Ca2+, Mg2+, Co2+, Mn2+, Sr2+, and Ba2+ were compared for their stimulatory or inhibitory effect on PG formation in rabbit kidney medulla slices. Calcium, manganese, and strontium ions stimulated PG generation up to 3- to 5-fold in a time- and dose-dependent manner (Ca2+ greater than Mn2+ approximately Sr2+) while the magnesium and cobalt ions were without significant effect...

Accumulation of Li+ in Saccharomyces cerevisiae X2180-1B occurred via an apparent stoichiometric relationship of 1:1 (K+/Li+) when S. cerevisiae was incubated in the presence of 5 and 10 mM LiCl for 3 h. Other cellular cations (Mg2+, Ca2+ and Na+) did not vary on Li+ accumulation, although lithium chemistry dictates a degree of similarity to Group I and II metal cations. Compartmentation of Li+...

Transition of leukocyte function-associated antigen-1 (LFA-1), from an inactive into an activate state depends on the presence of extracellular Mg2+ and/or Ca2+ ions. Although Mg2+ is directly involved in ligand binding, the role of Ca2+ in LFA-1 mediated adhesion remained obscure. We now demonstrate that binding of Ca2+, but not Mg2+, directly correlates with clustering of LFA-1 molecules at t...

Phosphatidate phosphohydrolase (PAP) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 U/mg protein. An activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using Ferguson plot. Molecular Weight (M.W.) of the active PAP was 298 KDa. SDS-PAGE analysis showed a M.W. of 47 KDa for...

A sensitive radioactive assay of acyl CoA:sn-glycerol3-phosphate-0-acyltransferase (EC 2.3.1.15) was developed to study the properties and subcellular distribution of this enzyme in rat epididymal adipose tissue. The esterification of sn-glycerol-3-phosphate was measured in the presence of palmitoyl CoA or palmitate, ATP, CoA, and Mg2+ at pH 7.5. The presence of glycerophosphate acyltransferase...