We examined whether non-enzymatic antioxidant defense systems are disrupted in the brain of rats with water-immersion restraint stress. When rats were exposed to water-immersion restraint stress for 1.5, 3 or 6 h, the brain had decreased ascorbic acid and reduced glutathione contents and increased lipid peroxide and nitric oxide metabolites contents at 3 h and showed further changes in these co...

BACKGROUND Although ACE inhibitors can protect myocardium against ischemia/reperfusion injury, the mechanisms of this effect have not yet been characterized at the cellular level. The present study was designed to examine whether an ACE inhibitor, cilazaprilat, directly protects cardiac myocytes against hypoxia/reoxygenation (H/R) injury. METHODS AND RESULTS Neonatal rat cardiac myocytes in p...

BACKGROUND When solutions of multivitamin preparations (MVPs) are exposed to light, H(2)O(2) as well as organic peroxides are generated and the concentration of vitamin C decreases. The aim of this study was to determine, using mass spectrometry, whether the generation of oxidative byproducts of vitamin C, such as dehydroascorbate (DHA) and 2,3-diketogulonic acid (DKG), accounted for the report...

Purpose: To determine the effect of the temperature of water used for the preparation of paracetamol and ascorbic acid mixture on its stability, as well as to assess the influence of humidity on the stability of single components and their mixtures. Methods: The stability of the mixtures in aqueous medium was evaluated with the aid of UV–Vis spectrophotometer interfaced with a computer. Spectra...

Methylene blue staining method was used to distinguish O. viverrini eggs from Haplorchis taichui and Prosthodendrium molenkampi eggs. All eggs were obtained from dissected adult worms, fixed in 10% formalin, and stained with methylene blue prior to light microscopy observation. The distinct musk-melon-like texture of the O. viverini eggshell surface and the thread-like texture of H. taichui egg...

The spinal cord injury (SCI) is a detrimental neurological disease involving the primary mechanical injury and secondary inflammatory damage. Curtailing the detrimental neuroinflammation would be beneficial for spinal cord function recovery. Microglia reside in the spinal cord and actively participate in the onset, progression and perhaps resolution of post-SCI neuroinflammation. In the current...

OBJECTIVES Vasoplegia is a frequent complication after cardiopulmonary bypass that often requires the application of norepinephrine. In a number of cases, however, vasoplegia is refractory to norepinephrine. The guanylate cyclase inhibitor methylene blue could be an attractive treatment alternative in such cases. This study examines the results of methylene blue therapy for norepinephrine-refra...

Dehydro-L-ascorbic acid, the oxidation product of L-ascorbic acid, is unstable in aqueous solution and decomposed by a hydrolytic disruption of its ring structure to 2,3-diketo-L-gulonic acid. It is shown by means of UV, 'H-, and 13C-NMR spectroscopy that after decarboxylation 3,4,5-trihydroxy-2-keto-L-valeraldehyde, an a-ketoaldehyde, is formed. This substance is oxidized further to L-erythroa...

In vitroantimicrobial activity, DNA cleavage, anti-diabetic, antioxidant, anti-inflammotary and antihaemolytic of some synthesized Metallocephradines and Metallocefepimes were examined. In a broadspectrum of antibacterial and antifungal property, it was found that nickel (1:1) and copper (1:2) complexes were more active than cephradine against all tested bacteria except S.pyogenes and E.fecalis...