نتایج جستجو برای: methylation specific pcr msp

تعداد نتایج: 1212906  

2015
XIAOYU FU XIAOLING SONG DEMING TAN GUOZHEN LIU

The aim of the present study was to investigate the effect of hepatitis B virus X protein (HBx) on the expression of DNA methyltransferase (DNMT)3A/3B and suppressors of cytokine signaling-1 (SOCS-1), as well as promoter CpG island methylation of the SOCS-1 gene. Stable hepatocyte cell lines expressing the HBx gene (pcDNA-X/QSG7701) or an empty gene (pcDNA3.0/QSG7701) were established. Reverse ...

2017
Zhisen Shen Chongchang Zhou Jinyun Li Dong Ye Hongxia Deng Bin Cao Wenjuan Hao Lexi Lin Yuna Zhang

The purpose of this study was to evaluate the contribution of SHISA3 promoter methylation to laryngeal squamous cell carcinoma (LSCC). SHISA3 promoter methylation status and expression were determined using methylation-specific polymerase chain reaction (MSP) and quantitative real-time PCR (qRT-PCR) in 93 paired LSCC and adjacent normal tissues, respectively. Furthermore, the regulatory functio...

Journal: :Clinica chimica acta; international journal of clinical chemistry 2015
Sophia Mastoraki Maria Chimonidou Lampros Dimitrakopoulos Sophia Kounelis Nikos Malamos Vassilis Georgoulias Evi Lianidou

INTRODUCTION SOX17 promoter methylation can provide important prognostic information in cancer. We developed a novel semi-quantitative MS-HRMA assay for SOX17 promoter methylation. METHODS The assay was optimized by using synthetic control samples and validated by analyzing 165 clinical samples: a) 107 formalin fixed paraffin embedded (FFPEs) samples of patients with early breast cancer, b) 2...

Journal: :Oncology reports 2010
Masatsugu Hiraki Yoshihiko Kitajima Yuji Nakafusa Jun Nakamura Kazuyoshi Hashiguchi Kenji Sumi Hirokazu Noshiro Kohji Miyazaki

Aberrant gene methylation is frequently observed in various cancers and plays an important role in carcinogenesis, cancer progression and drug responsiveness. The aim of this study is to identify colorectal cancer specific gene methylation determining chemosensitivity to S-1/CPT-11 therapy. The gene methylation of CHFR, p16, RUNX3, E-cadherin, MGMT, hMLH1, ABCG2, UGT1A1 and BNIP3 genes were ana...

2014
Ying-Li Lin Pei-Gen Xie Li Wang Jian-Guo Ma

BACKGROUND Aberrant methylation of protocadherin 17 (PCDH17) has been reported in several human cancers. However, the methylation status of PCDH17 in prostate cancer and its clinical significance remains unclear. The aim of this study was to investigate the methylation status of PCDH17 and its clinical significance in patients with prostate cancer after radical prostatectomy. MATERIAL/METHODS...

2015
Bo Wu Xiaoming Cao Xuezhi Liang Xuhui Zhang Wei Zhang Guang Sun Dongwen Wang

E74-like factor 5 (Elf5) has been associated with tumor suppression in breast cancer. However, its role in urothelial cancer (UC) is completely unknown. Immunohistochemistry (IHC) and methylation specific PCR (MSP) were done to detect Elf5 expression level and its promoter methylation. Results revealed that low expression of Elf5 on protein and mRNA levels were associated with tumor progression...

2011
Lihong Zhang Jing Shi Li Xu Bingyin Shi Peng Hou Meiju Ji

The genes encoding drug-metabolizing enzymes and transporters play an important role in maintaining the normal life processes of human body. Their disorder or defect will lead to the occurrence and development of various diseases. Currently, most of studies have focused on genetic variations in these genes, however, in the present study, we analyzed promoter methylation of 11 drug metabolism an...

ژورنال: پژوهش در پزشکی 2018

Title: Evaluation of expression and methylation of promoter of JAK2 and STAT3 genes in lung cancer tissue and its comparison with adjacent healthy tissue in 75 people with this cancer.             Objective: Lung cancer is a disease that affects lung tissue cells. It is a common cancer in the world and is one of the deadliest cancers. Molecular variations of genes have a significant effect...

2016
Wen-rui Wu Hong Sun Rui Zhang Xian-huan Yu Xiang-de Shi Man-sheng Zhu Hong Zeng Li-xu Yan Lei-bo Xu Chao Liu

This study aims to investigate the biological function of microRNA-200b and BMI1, predicted target of microRNA-200b in human hepatocellular carcinoma (HCC). MicroRNA-200b and BMI1 expression in HCC tissues were evaluated by qPCR. A luciferase reporter assay was used to validate BMI1 as a direct target of microRNA-200b. The effect of microRNA-200b on HCC progression was studied in vitro and in v...

2013
Kwan-Yeung Wong Rita Lok-Hay Yim Yok-Lam Kwong Chung-Ying Leung Pak-Kwan Hui Florence Cheung Raymond Liang Dong-Yan Jin Chor-Sang Chim

BACKGROUND MIR129-2 has been shown to be a tumor suppressor microRNA hypermethylated in epithelial cancers. PATIENTS AND METHODS Epigenetic inactivation of MIR129-2 was studied by methylation-specific PCR (MSP) in 13 cell lines (eight myeloma and five lymphoma), 15 normal controls and 344 primary samples including acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), chronic myelo...

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