Both the frequency and intensity of drought events are expected to increase, with unresolved alterations peatland methane cycling involved microbial communities. While existing studies have assessed effects via experimental approaches under controlled conditions, our knowledge, no examined in-situ natural in restored temperate fens. In this study, we used quantitative polymerase chain reaction ...

A new genus and species of a nonmotile gram-negative rod, Syntrophobacter wolinii, is the first bacterium described which degrades propionate only in coculture with an H(2)-using organism and in the absence of light or exogenous electron acceptors such as O(2), sulfate, or nitrate. It was isolated from methanogenic enrichments from an anaerobic municipal sewage digestor, using anaerobic roll tu...

The methanogenic community in hydrothermally active sediments of Guaymas Basin (Gulf of California, Mexico) was analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase (mcrA) and 16S rRNA genes. Members of the Methanomicrobiales and Methanosarcinales dominated the mcrA and 16S rRNA clone libraries from the upper 15 cm of the sediments. Within the H2/CO2- and format...

To understand the physiological basis of methanogenic archaea living on interspecies H(2) transfer, the protein expression of a hydrogenotrophic methanogen, Methanothermobacter thermautotrophicus strain ΔH, was investigated in both pure culture and syntrophic coculture with an anaerobic butyrate oxidizer Syntrophothermus lipocalidus strain TGB-C1 as an H(2) supplier. Comparative proteomic analy...

High roughage diet causes more methane emissions; however, the total methanogen abundance is not influenced by roughage proportion. Technologies to reduce methane emissions are lacking, and development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on present knowledge of the methanogens. In this work, we have investigated molecular diversity of r...

Copyright © 2016 by The Geochemical Society of Japan. globally deposited methane hydrate is estimated to be ~100,000 Gt (Kvenvolden, 2002; Milkov, 2004), which is a potent energy resource. Since the major source of methane in the hydrate is of methanogen origin, investigation of distribution and activities of methanogens leads to deeper understanding of the starting point of methane hydrate for...

The metagenomic whole genome shotgun sequencing (mWGS) approach was used to detect signatures of the rhizosphere microbiomes Dipterygium glaucum and surrounding bulk soil microbiomes, differential microbial responses due watering. Preliminary results reflect reliability experiment rationality grouping microbiomes. Based on abundance non-redundant genes, bacterial genomes showed highest level, f...

Abstract. Understanding diagenetic reactions in accreted sediments is critical for establishing the balance of fluid sources and sinks accretionary prisms, which turn important assessing pressure field ability faults to host seismic slip. For this reason, we studied deformation bands (shear zones veins) within deep mud from Nankai prism (SW Japan) drilled at site C0001 during IODP Expedition 31...