detection of chikungunya virus using a RT-PCR/nested PCR combination .opment of a TaqMan RT-PCR assay without RNA extraction step for the detection and quantifi cation of African chikungunya viruses. To the Editor: Respiratory viral infections can be associated with a wide range of clinical manifestations from self-limiting upper respiratory tract diseases to pneumonia (1). However, in general,...

BALB/c mice infected with human metapneumovirus (hMPV) were treated with the sulfated sialyl lipid NMSO3 (one dose of 50 mg kg(-1)) given at the time of infection. NMSO3 significantly reduced viral replication in the lungs, as well as hMPV-induced body weight loss, pulmonary inflammation and cytokine production, suggesting that antiviral treatment initiated at the beginning of viral infection c...

280 Characterization of infectious bronchitis virus isolates from backyard flocks. AAAP abstract † 281 Recombination in avian gamma-coronavirus infectious bronchitis virus (IBV). 282 Evaluation of possible interference between Arkansas and Massachusetts vaccine serotypes. AAAP abstract † 283 Interactions between multivalent attenuated live infectious bronchitis virus (IBV) vaccines in one-day-o...

Paramyxovirus spread generally involves assembly of individual viral particles which then infect target cells. We show that infection of human bronchial airway cells with human metapneumovirus (HMPV), a recently identified paramyxovirus which causes significant respiratory disease, results in formation of intercellular extensions and extensive networks of branched cell-associated filaments. For...

New derivatives of synthetic 5,5-diphenylhydantoin (phenytoin) were prepared by N-alkylation with 1,3-dibromopropane. Subsequent treatment with sodium azide led to the respective azide. Reaction of the azide with phenylacetylene and 2-hydroxy-3-butyne and oxidation of the resulting alcohol with MnO2 resulted in three triazolic compounds that were evaluated in vitro for their antiviral activity ...

BACKGROUND The use of cell culture for the diagnosis of human metapneumovirus (hMPV) infection is uncommon at present and molecular method such as reverse-transcription PCR (RT-PCR) has been widely and most commonly used as the preferred test. We aimed to compare the results of virus isolation using Vero E6 cells with real-time RT-PCR for the detection of hMPV, since such a comparison data is n...

Human respiratory syncytial virus (HRSV) and human metapneumovirus (HMPV) are ubiquitous respiratory pathogens of the Pneumovirinae subfamily of the Paramyxoviridae. Two major surface antigens are expressed by both viruses; the highly conserved fusion (F) protein, and the extremely diverse attachment (G) glycoprotein. Both viruses comprise two genetic groups, A and B. Circulation frequencies of...

BACKGROUND In 2006, bronchiolitis due to adenovirus nosocomial infections resulted in the closure of a pediatric department in northern Portugal. OBJECTIVES To determine the etiology of bronchiolitis in northern Portugal. STUDY DESIGN It was a prospective multicenter study on the etiology of bronchiolitis during the respiratory syncytial virus (RSV) season (November-April). Children < or = ...