Three psychrophilic protein pheromones (En-1, En-2 and En-6) from the polar ciliate, Euplotes nobilii, and six mesophilic pheromones (Er-1, Er-2, Er-10, Er-11, Er-22 and Er-23) from the temperate-water sister species, Euplotes raikovi, were studied in aqueous solution for their thermal unfolding and refolding based on the temperature dependence of their circular dichroism (CD) spectra. The thre...

The stability of two enzymes from extreme thermophiles (glutamate dehydrogenase from Thermococcales strain AN1 and beta-glucosidase from Caldocellum saccharolyticum expressed in Escherichia coli) has been exploited to allow measurement of activity over a 175 degrees C temperature range, from +90 degrees C to -85 degrees C for the glutamate dehydrogenase and from +90 degrees C to -70 degrees C f...

INTRODUCTION The aim of study was to estimate the qualitative and quantitative composition of moulds colonizing sunflower seeds on RBCA, YpSs, DG18 media at 25, 37, and 45°C. MATERIAL Twenty samples of sunflower seeds (not roasted and not salted) were used for the analysis. The highest number of moulds were xerophilic species isolated on DG18 at 25°C (33), followed by mesophilic species (29) ...

The aim of this study was to evaluate microbiological contamination levels of indoor air in some farm settings, including barns and dairy objects. The level of microorganism emissions from farm buildings into atmospheric air was also estimated. In the research a MAS-100 air sampler (MERCK) was used. It was revealed that the number of microorganisms in barns ranged between 1.7·10 8.8·10 for meso...

Roth, Norman G. (Whirlpool Corp., St. Joseph, Mich.) and Robert B. Wheaton. Continuity of psychrophilic and mesophilic growth characteristics in the genus Arthrobacter. J. Bacteriol. 83:551-555. 1962.-The effect of temperature on growth of seven members of the genus Arthrobacter was determined at 0, 7, 20, 30, and 37 C. In general, no sharp cutoff point was observed between growth-temperature r...

The temperature dependence of the dynamics of mesophilic and thermophilic dihydrofolate reductase is examined using elastic incoherent neutron scattering. It is demonstrated that the distribution of atomic displacement amplitudes can be derived from the elastic scattering data by assuming a (Weibull) functional form that resembles distributions seen in molecular dynamics simulations. The thermo...

Thermotropic properties of blue-green algae grown at high, room, and low temperatures in H(2)O and D(2)O media were studied by highly sensitive differential scanning microcalorimetry. The thermograms of these organisms contain an endothermal peak in the temperature range of 50 to 70 C with an endothermal heat ranging from 0.14 to 1.91 joules per gram organism. The temperature at which the endot...

It has been known for some time that thermophilic proteins generally have increased numbers of non-covalent interactions (salt bridges, hydrogen bonds, etc.) compared with their mesophilic orthologs. Recently, anecdotal structural comparisons suggest that non-specific acid-base ion pairs on the protein surface can be an evolutionary efficient mechanism to increase thermostability. In this compr...

Microbial diversity in a thermopilic UASB reactor producing biogas from palm oil mill effluent (POME) was investigated. The PCR-based DGGE analysis showed that the microbial population profiles changed with the temperature transition from mesophilic to thermophilic conditions. Acetotrophic and hydrogenotrophic methanogens were found in all three phases: the seed sludge, transition from mesophil...