In a recent account of methods of obtaining best-fit parameters of the parameters V and Km of the Michaelis-Menten equation, Tseng & Hsu (1990) appear to have misunderstood the method used by Lineweaver & Burk (1934), and to have overlooked much of the work done since 1934. The Michaelis-Menten equation represents the observed rate of v of an enzyme-catalysed reaction in terms of the two parame...

We report a general kinetics model for catalytic dioxygen reduction on multicopper oxidase (MCO) cathodes. Our rate equation combines Butler-Volmer (BV) electrode kinetics and the Michaelis-Menten (MM) formalism for enzymatic catalysis, with the BV model accounting for interfacial electron transfer (ET) between the electrode surface and the MCO type 1 copper site. Extending the principles of MM...

The kinetic mechanism of enzymatic modification of flavonol quercetin with L-cysteine by horseradish peroxidase (HRP) was studied. Reaction of modification of quercetin was followed by recording spectral changes over time at 380 nm. All reactions were performed in 100 mM phosphate buffer pH, 6.0 at 20 degrees C. Kinetic parameters were determined from graphics of linear Michaelis-Menten equatio...

We study the convergence rate of MCMC on the statistically unidentifiable nonlinear model involving the Michaelis-Menten kinetic equation. We have shown that, under certain conditions, the convergence diagnosis of Raftery and Lewis (1992) is consistent with the convergence rate argued by Brooks and Roberts (1999). Therefore, different MCMC schemes developed in linear models are further extended...

Jacobsen CF, Leonis J, Linderstr0m-Lang K, Ottsen M. The pH-stat and its use in biochemistry. Methods Biochem Anal 1957;4:171-210. 2 Heller M, Hanahan DJ. Human erythrocyte membrane bound enzyme: acetylcholinesterase. Biochim Biophys Acta 1972;255:251-72. 3 Newman PFJ. A study of acetylcholine acetyl-hydrolase in the human erythrocyte. MPhil thesis, Edinburgh University, 1974. 4 Baggott J. The ...

Mixed-microbial assemblages enriched from a septic tank, coastal sediment samples, the digester sludge of a brewery wastewater treatment plant and acidic sulfate soil samples were compared on the basis of growth rate, waste and sulfate reduction rate under sulfate reducing conditions at 30 degrees C. The specific growth rate of various cultures was in the range 0.0013-0.0022 hr(-1). Estimates o...

Abstract Mitochondria is a decisive organelle of cells that produces adenosine triphosphate (ATP) by the process oxidative phosphorylation Krebs cycle and electron transport chain. The chain system mitochondria embodies multiple enzyme supercomplexes including complex I to V which located in inner membrane. Although simple activity some as-isolated has been studied so far, steady-state kinetic ...