Tissue factor (TF), an initiator of blood coagulation in vivo, is expressed in a variety of cells. Sufficient natural TF has been isolated to clone and express recombinant proteins ranging from full-length TF to its extracellular domain. Because of the limited availability of natural TF, recombinant proteins have been used as surrogates. Despite the differences in their post-translational modif...

Human leukocyte antigen-G (HLA-G) is a low polymorphic nonclassical HLA-I molecule restrictively expressed and with suppressive functions. HLA-G gene products are quite complex, with seven HLA-G isoforms, four membrane bound, and other three soluble isoforms that can suffer different posttranslational modifications or even complex formations. In addition, HLA-G has been described included in ex...

This paper compares the principal modifications taking place in spermatozoa during epididymal transit in several species. The relations between changes in the epididymal medium and modifications in the metabolism, motility and/or fertility of spermatozoa are reviewed with particular reference to spermatozoon forward motility. The fertilizing ability of motile testicular and epididymal spermatoz...

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins at the cell surface. The application of proteomic approaches to the cell wall compartment raises important questions: are there technical problems specific to cell wall proteomics? What kinds of pr...

Surface modification of polyethersulfone (PES) membrane surfaces using UV lozone pretreatment with sub­ sequent grafting and interfacial polymerization on membrane surface was investigated in order to improve the resistance of membrane surface to protein adsorption. The surface modifications were evaluated in terms of hydrophilicity, chemical composition of the surface and static protein adsorp...

The ABCA1 transporter organizes cellular lipid homeostasis by promoting the release of cholesterol to plasmatic acceptors such as ApoA-I. Despite intensive investigation, the molecular mechanism of such a process has not yet been clarified. In the present study we report on the analysis of the ABCA1 lipid microenvironment at the plasma membrane of living cells, by a novel approach based on fluo...

Differentiated thyroid cancer is the most common malignancy of endocrine system. Although nodules are benign, given high incidence in population, it important to understand differences between benign and malignant molecular alterations associated with improve detection signal potential diagnostic, prognostic, therapeutic targets. Proteomics analysis human tissue largely revealed changes indicat...