In this study, the separation parameters in micellar electrokinetic chromatography (MEKC)-laser-induced fluorescence (LIF) were optimized for the separation of red and yellow historical dyes in 20 mM borate buffer with 20 mM sodium dodecyl sulfate (SDS). Separation conditions were optimized by changing pH, organic modifier (methanol and acetonitrile) concentrations and applied voltage. The mixt...

A simple capillary zone electrophoresis (CZE) method was used to characterize human very low-density lipoprotein (VLDL) particles for four healthy donors. One major peak was observed for native, in vitro oxidized and glycated VLDL particles. The effective mobilities and peak areas of the capillary electrophoresis (CE) profiles showed good reproducibility and precision. The mobility of the oxidi...

A stable polyelectrolyte multilayer (PEM) coating was investigated for use in open-tubular capillary electrochromatography (o-CEC). In this approach, the PEM consisted of the cationic polymer of a quaternary ammonium salt, poly(diallyldimethylammonium chloride) and the anionic polymeric surfactant, poly(sodium undecylenic sulfate). Both the cationic and anionic polymers were physically adsorbed...

In this paper, a multiresidue method for the analysis in soils of metribuzin (M) and its major conversion products, deaminometribuzin (DA), diketometribuzin (DK) and deaminodiketometribuzin (DADK) is developed. Considering the neutral and charged nature of the molecules, micellar electrokinetic chromatography (MEKC) is a very efficient method for the separation of these compounds, providing hig...

Fluorescent probes enable detection of otherwise nonfluorescent species via highly sensitive laser-induced fluorescence. Organic amines are predominantly nonfluorescent and are of analytical interest in agricultural and food science, biomedical applications, and biowarfare detection. Alexa Fluor 488 N-hydroxysuccinimidyl ester (AF488 NHS-ester) is an amine-specific fluorescent probe. Here, we d...

This paper reports an fluorescence imaging analysis to verify the proposed mechanisms in transient trapping– microchip micellar electrokinetic chromatography (tr-trapping–MCMEKC). Fluorescence imaging revealed that a process of tr-trapping consisted of four migration stages with different apparent diffusion constants. In the first trapping stage, the diffusion of a focused sample band was suppr...

A microwave-assisted fluorescence labeling method for use in CE-LIF (capillary electrophoresis-laser induced fluorescence) is described. Six amphetamine-like designer drugs, namely, o-, m-, p-chloro- and o-, m-, p-fluoro-amphetamine derivatives, were synthesized and used as model compounds. FITC (fluorescein isothiocyanate isomer I) and a blue-laser were used as the fluorescent labeling reagent...

We developed moment analysis of affinity kinetics by chromatographic capillary electrophoresis (MKCCE) method for the kinetic study of intermolecular interactions. Association and dissociation rate constants of the interaction in a micellar electrokinetic chromatography (MEKC) system between thymol and sodium dodecylsulfate micelle were determined by the MKCCE method. It is a method based on th...