BACKGROUND The unfolded protein response (UPR) is an intracellular signaling pathway that is activated by the accumulation of unfolded proteins in the endoplasmic reticulum (ER). The UPR results in an increase in transcription of ER-resident proteins that facilitate protein folding in the ER. A key regulatory step in UPR activation is the regulated splicing of HAC1 mRNA, which encodes Hac1p, a ...

The mRNA endonuclease PMR1 initiates mRNA decay by forming a selective complex with its translating substrate mRNA. Previous work showed that the ability of PMR1 to target to polysomes and activate decay depends on the phosphorylation of a tyrosine residue at position 650. The current study shows that c-Src is responsible for activating this mRNA decay pathway. c-Src was recovered with immunopr...

BackgroundMast cells (MCs) are key regulators of IgE-mediated allergic inflammation. Cell-derived extracellular vesicles (EVs) contain bioactive compounds such as microRNAs. EVs can transfer signals to recipient cells, thus using a novel mechanism cell-to-cell communication. However, whether MC-derived involved in FcεRI-mediated inflammation is unclear.ObjectiveWe sought investigate the effect ...

Analogous to DNA and histone modifications, RNA molecules are chemically modified, the study of which gives rise field called epitranscriptomics. Among these N6-methyladenosine (m6A) methylation is most abundant internal modification in messenger (mRNA) non-coding eukaryotic cells, regulates metabolism, including decay, translation nuclear processing. However, regulatory functional role m6A ski...