Objective Aspirated ovarian follicular fluids (FF) contain luteal granulosa cells (LGC) and other contaminating cell types. Several strategies such as the antibody binding methods, the flask method, the cell strainer, and the positive selection of granulosa aggregates after density gradient centrifugation, were tested as LGCs purification methods. Each of these strategies has its own advantages...

An electricity-generating bacterium, Geobacter sulfurreducens PCA, was inoculated into a single-chamber, air-cathode microbial fuel cell (MFC) in order to determine the maximum electron transfer rate from bacteria to the anode. To create anodic reaction-limiting conditions, where electron transfer from bacteria to the anode is the rate-limiting step, anodes with electrogenic biofilms were reduc...

objective(s) mesenchymal stem cells (mscs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. the present study tried to isolate and characterize mscs from goat bone marrow. materials and methods fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. passaged-3 cells were then differentiat...

purpose: to compare the clinical and paraclinical outcomes following deep anterior lamellar keratoplasty (dalk) using frozen versus fresh corneas in patients with keratoconus. methods: in this prospective, comparative interventional study, 16 frozen and 19 fresh corneas were used during dalk in keratoconus patients. big bubble technique was used in all patients. the two groups were compared at ...

According to the Narnhofer Thirring Theorem [1] interacting systems at finite temperature cannot be described by particles with a sharp dispersion law. It is therefore mandatory to develop new methods to extract particle masses at finite temperature. The Maximum Entropy method offers a path to obtain the spectral function of a particle correlation function directly. We have implemented the meth...

The data of the experiment of Schiller et al., Phys. Rev. Lett. 77(1996) 2933, are alternatively evaluated using the maximum likelihood estimation. The given data are fitted better than by the standard deterministic approach. Nevertheless, the data are fitted equally well by a whole family of states. Standard deterministic predictions correspond approximately to the envelope of these maximum li...

We calculate the density profiles of virialized halos in the case of structure evolving hierarchically from a scale-free Gaussian δ−field having a power spectrum P (k) ∝ kn in a Ω = 1 Universe; we suppose that the initial density contrast profile around local maxima is given by the mean peak profile introduced by Bardeen et al. (1986 hereafter BBKS). We show both that the density profiles are n...