Background This study aimed to investigate whether Artificial Seminiferous Tubules (AST) coated with testicular extra cellular matrix (ECM) support maturation of spermatogenic cells from immature testes. MaterialsAndMethods Medical grade tubes coated with testicular ECM were used. Testes from immature mice were loaded in the concentration of 10000- 20000/ml. Tubes were then immersed in germ cel...

Cytochrome P450 proteins belong to one of the largest families enzyme in plants and play important roles plant growth development stress response. In our previous studies, a cytochrome gene, GhCYP94C1 (cytochrome 94C1), was functionally characterized as positive regulator seed germination, main root elongation early flowering. However, whether gene has other potential functions rem...

Background: Dendritic cells (DC) induce tumor or pathogen-specific T cell responses in humans. Several laboratories have developed culture systems, including maturation factors for human DC from peripheral blood monocytes. We comprehensively compared standard maturation stimulus, an autologous monocyte-conditioned medium (MCM), with heparin for their ability to promote uniformly mature DC that ...

Knowledge about gene expression during sugarcane growth and maturation is limited. A gene discovery programme has been in progress at SASEX since 1994 to identify and analyse genes that are expressed in different sugarcane tissue types and developmental stages. A database of sugarcane gene sequences has been established through the generation of Expressed Sequence Tags (ESTs). This database, co...

sea cucumbers saponins are triterpenoid glycosides which exert beneficial biomedical effects. this study was performed to assess the effect of saponin extracted from sea cucumber holothuria leucospilota            ( h. leucospilota ) on maturation of mice oocytes and granulosa cells. the germinal vesicles oocytes were collected from 6–8 weeks old naval medical research institute (nmri) mice ova...

Endogenous abscisic acid (ABA) levels are regulated by both biosynthesis and catabolism of the hormone. ABA 8'-hydroxylase is considered to be the key catabolic enzyme in many physiological processes. We have previously identified that four members of the Arabidopsis (Arabidopsis thaliana) CYP707A gene family (CYP707A1 to CYP707A4) encode ABA 8'-hydroxylases, and that the cyp707a2 mutants showe...

dendritic cells (dcs) induce pathogen-specific t cell responses. we comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), pha activated t cells (t cell conditioned medium), and mixture of fibroblast & pha activated t cells (fcm-tccm) conditioned media on maturation of dcs. monocytes were cultured with gm-csf and il-4 for five da...

Altered profiles of gene expression reflect the reprogramming of intestinal epithelial cells during their maturation along the crypt-luminal axis. To focus on genes important in this process, and how they in turn are regulated, we identified 14 transcripts commonly downregulated in expression during lineage-specific maturation of the immortalized cell lines Caco-2 (absorptive), HT29Cl16E (goble...

TISSUE-SPECIFIC CHANGES in gene expression during maturation may provide important insights into tissuespecific gene expression patterns during pathogenesis. In this context, the cellor tissue-surrounding environment or stroma has been recognized as an important regulator (11). The great diversity observed in the composition and morphology of the extracellular environment contributes significan...