We report the analysis and characterization of natural and modified oligonucleotides by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The present technology was highly improved for this class of compounds by using a new matrix, 2,4,6-trihydroxy acetophenone, together with di- and triammonium salts of organic or inorganic acids to suppress peak broa...

Abstract This study focused on characterization of Volatile Organic Compounds (VOCs) as contaminants and non-volatile additives in Italian PET bottles, also suggesting potential functional aldehydes scavengers. Several VOCs, such acetic aldehyde (AA), butanal, 3-methyl 1,3-dioxolane, pentanal, hexanal, octanal, 5-hepten-2-one, nonanal, decanal, were identified by Head Space-Gas Chromatography/M...

Proteomics is the study of and the search for information about proteins. The development of mass spectrometry (MS) such as matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) MS and imaging mass spectrometry (IMS), greatly speeds up proteomics studies. At the same time, the MS and IMS applications in medical science give rise to many challenges in mathematics and statistic...

Glycomics is a developing field that provides structural information on complex populations of glycans isolated from tissues, cells and organs. Strategies employing matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) are central to glycomic analysis. Current MALDI-based glycomic strategies are capable of efficiently analyzing glycoprotein and glycosphingo...

Reported matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification rates of Gram-positive rods (GPR) are low compared to identification rates of Gram-positive cocci. In this study, three sample preparation methods were compared for MALDI-TOF MS identification of 190 well-characterized GPR strains: direct transfer, direct transfer-formic acid prep...

Circulating atypical cells (CAC) are released from a primary tumour site into peripheral blood and indicators of cancer metastasis. CAC occur at very low frequency in circulating blood, their detection remains challenging. Moreover, white (WBC) the major contaminant enriched samples. Here, we developed matrix-assisted laser desorption ionization–time flight mass spectrometry (MALDI-TOF MS) as n...

4-Hydroxy-2-nonenal (4HNE), a major secondary product of lipid peroxidation, has been associated with a number of disease states involving oxidative stress. Despite the recognized importance of post-translational modification of proteins by products such as 4HNE, little is known of the modification of cytochrome c by this reagent and its analysis by mass spectrometry. The purpose of this study ...

conclusions these results show the usefulness of proteome analysis in successful identification of the common proteins between n. lactamica strains and nmb. this proteomics analysis is the starting point in the path of knowledge development about whole proteome profiles of n. lactamica strains. objectives the main aims of this study were to determine whole proteome profiles of n. lactamica stra...

Angiotensin I, a model decapeptide, was glycosylated and partially hydrolyzed with HCl (6 N, 80 C, 4 h), aminopeptidase, and carboxypeptidase Y. A single peptide mass map obtained from truncated peptides in the partial acid hydrolysate of angiotensin and its glycosylation product mixture by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry enabled sequenci...

BACKGROUND Whole-cell matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been successfully applied for bacterial identification and typing of many pathogens. The fast and reliable qualities of MALDI-TOF MS make it suitable for clinical diagnostics. MALDI-TOF MS for the identification and cluster analysis of Streptococcus pyogenes, however, has not ...