BACKGROUND Trophinin is an intrinsic membrane protein that forms a complex in the cytoplasm with bystin and tastin, linking it microtubule-associated motor dynein (ATPase) in some cell types. Previously, we found that human sperm tails contain trophinin, bystin and tastin proteins, and that trophinin-binding GWRQ (glycine, tryptophan, arginine, glutamine) peptide enhanced motility of human sper...

Excess weight and obesity have become a serious problem in adult men of reproductive age throughout the world. The purpose of this retrospective study was to assess the relationships between body mass index and sperm quality in subfertile couples in a Chinese Han population. Sperm analyses were performed and demographic data collected from 2384 male partners in subfertile couples who visited a ...

We describe the use of interferometric microscopy coupled with a novel application of Sénarmont compensation for detecting and quantifying the distribution of dry matter in cultured cells. In conjunction with video techniques and digital image processing, a two-dimensional, calibrated map of the dry mass distribution in an isolated cell can be obtained and digitally recorded. We have called the...

background: sperm vitrification is a technique of ice and cryoprotectant free cryopreservation by direct plunging of sperm suspension into liquid nitrogen (ln2). the aim of this study was to investigate the influence of cryoprotectant free-vitrification on human sperm fine structure by msome technology and the fertility potential by zona binding assay (zba). methods: 20 normo-ejaculates were pr...

conclusions in conclusion, rsasl and rsacod differed for time variables (tt and bt) and hr responses. despite being non-significant, the trend that adding cod reduced the percentage differences in tt and bt between the two groups highlighted the role of sport specialization. results compared with rsasl, tt and bt were worst in rsacod (38.13 vs. 27.52 s and 3.67 vs. 2.66 s, p < 0.001, respective...

The cross-species bacterial communication signal autoinducer 2 (AI-2), produced by the purified enzymes Pfs (nucleosidase) and LuxS (terminal synthase) from S-adenosylhomocysteine, directly increased Escherichia coli biofilm mass 30-fold. Continuous-flow cells coupled with confocal microscopy corroborated these results by showing the addition of AI-2 significantly increased both biofilm mass an...

Objective: Obesity is associated with inflammatory process and many different diseases. The objective of this study was to assess the impact of short term aerobic training on serum resistin and insulin resistance in adult obese women. Materials and Methods: In this quasi-experimental study, thirty untrained adult obese females matched for age 35-45 years old with body mass index (BMI) 30-36 kg...

BACKGROUND/AIMS We assessed the bolus transit and motility characteristics in gastroesophageal reflux disease (GERD) patients with abnormal esophageal pH monitoring. METHODS We retrospectively reviewed the combined impedance-esophageal manometry data from consecutive patients who had abnormal acid exposure during 24-hour esophageal pH monitoring. We compared these data to the results from fun...

The aim of this study was to evaluate sperm quality following separation and selection using a silane-coated silica colloid method (PureSperm ® ) or Percoll ® . Frozen/thawed sperm from buffalo bulls (n=5) were assigned to two groups. The first group was separated using a Percoll ® discontinuous gradient (45 and 90%) and the second group by a single layer of PureSperm ® . Sperm motility, concen...

BACKGROUND The aim of this study was to investigate the effects of sleep duration and bedtime on sperm health, and the possible mechanism involved. MATERIAL AND METHODS We randomly divided 981 healthy Chinese men into groups according to research-set bedtimes (A=8-10 PM, B=after 10 PM, and C=after midnight) and sleep durations: group 1=<6.0 h (short), group 2=7.0-8.0 h (average), and group 3=>9...