Inland solar salterns established in the vicinity of Sambhar Lake are extreme saline environments with high salinity and alkalinity. In view of the fact that microbes inhabiting such extreme saline environments flourish the contemporary bioprospecting, it was aimed to selectively isolate slow growing and rare actinomycetes from the unexplored solar salterns. A total of 14 slow growing actinomyc...

the focus of this study was antimicrobial membrane-activity of actinomycetes isolated from soils of iran. in this work, soil samples were collected from desert and farming zones of northern and central iran. a total number of 45 actinomycetes were isolated from the soil samples. in the primary screening performed to evaluate antimicrobial activity, isolated microorganisms were analyzed in terms...

Simulated municipal solid waste (MSW) based on the actual proportional characteristics of MSW was loaded into a simulated leachate recirculation bioreactor landfill and the abundance of common and tolerant microbes exposed to dibutyl phthalate (DBP) were investigated in the initial, acidic and methanogenic phases. The results showed that the abundance of bacteria was greatest, while that of act...

HeLa cell-based cytological profiling (CP) was applied to an extract library of marine sediment-derived actinomycetes to discover new cytotoxic secondary metabolites. Among the hit strains, Streptomyces sp. CP26-58 was selected for further investigation to identify its cytotoxic metabolites. CP revealed that the known ionophore tetronasin (1) was responsible for the cytotoxic effect found in th...

Hyaluromycin (1), a new member of the rubromycin family of antibiotics, was isolated from the culture extract of a marine-derived Streptomyces sp. as a HAase inhibitor on the basis of HAase activity screening. The structure of 1 was elucidated through the interpretation of NMR data for the compound and its 3″-O-methyl derivative in combination with an incorporation experiment with [1,2-13C2]ace...

Monsanto’s Bt-cotton NC 33B, planted in northern China for more than one decade, effectively controls cotton bollworms; however the understanding of its potential effects on soil microorganisms is limited. The dynamics of eubacterial, fungal and actinomycetes population sizes in rhizosphere soil of the Bt cotton were analysed by realtime PCR (qPCR) at the different growth stages under field con...

Actinomycetes populate soils and aquatic sediments that impose biotic and abiotic challenges for their survival. As a result, actinomycetes metabolism and genomes have evolved to produce an overwhelming diversity of specialized molecules. Polyketides, non-ribosomal peptides, post-translationally modified peptides, lactams, and terpenes are well-known bioactive natural products with enormous ind...

The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in lead discovery. Here, we describe a process for leveraging the concentration-response curves obtained from quantitative HT...

[Objective] Screening and identification of a marine-derived actinomycetes with antifugal activity, and then to determine the optimum fermentation conditions. [Method] Candida albicans was used as indicator bacteria to screen active strains. Strain H74-21 was identified based on its morphological, cultural, physiological and sequence analysis of 16S rDNA. HPLC-MS was used to determine the optim...

Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that the strains are members of the genera Kocuria and Micrococcus. To assess their biosynthetic pote...