Aldose-6-phosphate reductase (alditol 6-phosphate:NADP 1-oxidoreductase) was isolated and characterized from mature apple leaves (Malus domestica cv. Starkrimson). The enzyme was purified 79-fold. The enzyme catalyzed the following reversible reaction: d-glucose 6-phosphate + NADPH + H(+) right arrow over left arrow d-sorbitol 6-phosphate + NADP(+). No activity was detected when NAD(+) was subs...

Herein, we report the first cloning, recombinant expression, and synthetic utility of a sugar nucleotidyltransferase from any archaeal source and demonstrate by an electrospray ionization mass spectrometry (ESI-MS)-based assay its unusual tolerance of heat, pH, and sugar substrates. The metal-ion-dependent enzyme from Pyrococcus furiosus DSM 3638 showed a relatively high degree of acceptance of...

Rabbit alveolar macrophages express a plasma-membrane receptor that recognizes glycoprotein ligands bearing terminal mannose, fucose or N-acetylglucosamine residues. Macrophage membranes were washed extensively with buffers containing high salt and mannose or EDTA to remove endogenously bound ligand, before Triton X-100 extraction. The extracts were chromatographed on mannose-Sepharose. Elution...

this investigation was designed to determine the effect of intact ovalbumin and mannose-conjugatedovalbumin on the prevention of salmonella typhimurium adherence to the epithelium of small intestine ofchickens. mannose-conjugated ovalbumin was produced by maillard-type reaction between chickenovalbumin and d-mannose at 60°c. the results revealed that incubation up to 96 hrs caused the highestam...

Treatment of rabbit aortic endothelial cells, human umbilical vein endothelial cells, and human aortic endothelial cells for 4 hours with minimally oxidized low-density lipoprotein (MM-LDL) induced the adhesion of monocytes but not neutrophils or lymphocytes to these cells. This induction was blocked by inhibitors of glycoprotein synthesis (cycloheximide and tunicamycin), and binding was abolis...

Cathepsin D is an important lysosomal aspartic protease and is transported to the lysosomes in both mannose 6-phosphate dependent and independent manner. The present study reports lysosomal transport and proteolytic characterization of cathepsin D purified from gonads of starfish Asterias Rubens. Activity of the purified enzyme was confirmed by a zymogram assay on hemoglobin. Characterization o...