micrornas (mirnas) are short rna molecules which bind to target mrnas, resulting in translational repression and gene silencing and are found in all eukaryotic cells. approximately 2200 mirna genes have been reported to exist in the mammalian genome, from which over 1000 belong to the human genome. many major cellular functions such as development, differentiation, growth, and metabolism are kn...

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In article number 2000307, Tim Blomeier and co-workers describe the Blue Light-Operated CRISPR/Cas13b-mediated mRNA Knockdown. “Lockdown” introduces optical control of RNA levels utilizing a blue light-dependent switch to induce expression CRISPR/Cas13b, which mediates sequence-specific knockdown exo- endogenous mRNA. Further, they combined tool with other optogenetic switches for efficient dow...

Abstract Timely and accurate duplication of DNA prior to cell division is a prerequisite for propagation the genetic material both daughter cells. synthesis initiates at discrete sites, termed replication origins, proceeds in bidirectional manner until all genomic replicated. Despite fundamental nature these events, uniform method that identifies origins comprehensive still missing. Here, we pr...

Stem cells are unique that have the ability to differentiate into with specific functions and renew themselves produce new stem cells. One type of actively studied is Spermatogonial Cells (SSC). SSC a unipotent cell develops spermatozoa in mammalian testis. can be transplanted male testis recipients improve genetic quality livestock propagate valuable livestock. transplantation procedure starte...

Background: Microtubules (MT) are important components of cell cytoskeleton and play key roles in cell motility mitosis and meiosis. They are also the targets of several anticancer agents which indicating their importance in maintaining cell viability. Microtubular reorganization contributing to apoptotic morphology occurs in normal and neoplastic cells undergoing apoptosis induced by cytotoxic...

Spermatogonial stem cells (SSCs) also known as germ stem cells (GSCs) are the basis of spermatogenesis process in the testis. Furthermore, they are also valuable cells with different applications in developmental biology, transgenesis technology, and clinic. Understanding the new findings related to the cell and molecular biology of SSCs and the methods for isolation and maintenance of these ce...

To explore the mechanism of microRNA-125a reversing resistance laryngeal cancer stem cells to cisplatin from mitochondrial autophagy. The human carcinoma cell line Hep-2 was purchased Shanghai Bioleaf Biotechnology Co., Ltd. expression messenger RNA in each group analyzed by reverse transcription-polymerase chain reaction. migration and invasion ability evaluated Transwell assay. Cell apoptosis...

It is well established that the adult mammalian pineal body (PB), with exception of rodents, contains nerve cell bodies. Based on our previous results we have proposed there a pinealo-to-retinal neuronal connection in hamsters and prebubertal rats. By time animals reached puberty, labeled cells PB were not observed In present experiment, provide light electron microscopic immunohistochemical ev...