The mouse mutant hemoglobin deficit (gene symbol hbd) is characterized by a severe microcytic anemia that is inherited in an autosomal-recessive manner. To assess the mutation's effect on hematopoiesis, unfractionated bone marrow (BM) from either a mutant C57BL6/J-hbd/hbd, Gpi1b/Gpi1b (phenotype symbol HBD), or normal C57BL6/J-+hbd/+hbd, Gpi1b/Gpi1b mouse was injected intravenously into irradia...

There has been significant utilization of the technique described by Hamelmann et al. (1997) in which a parameter, Penh, related to airways responsiveness is non-invasively measured using unrestrained plethysmography (UP). We investigated this technique, seeking to answer these questions: 1) How do changes in Penh compare with changes in traditional plethysmographic and lung mechanical paramete...

BACKGROUND The classical renin-angiotensin system is known as the angiotensin (Ang)-converting enzyme/Ang II/Ang type 1 receptor axis, which induces various organ damage including cognitive decline. The angiotensin-converting enzyme 2/Ang-(1-7)/Mas axis is known to exert antagonistic actions against the classical renin-angiotensin system axis in the cardiovascular system. However, its roles in ...

A positive relationship between obesity and asthma has been well documented. The AMP-activated protein kinase (AMPK) activator metformin reverses obesity-associated insulin resistance (IR) and inhibits different types of inflammatory responses. This study aimed to evaluate the effects of metformin on the exacerbation of allergic eosinophilic inflammation in obese mice. Male C57BL6/J mice were f...

Abstract Background Inflammatory bowel disease is a chronic and excessive inflammation of the colon small intestine. We previously reported that priming mesenchymal stromal cells (MSCs) with poly(I:C) induced them to express indoleamine 2,3-dioxygenase (IDO). tried find out whether IFN-γ poly(I:C)-primed MSCs have better therapeutic efficacy on experimental colitis in IDO1-dependent manner. Met...

area burn (C57BL6 mice; n=4 per time point). Imaging of the injured limb and skin incision only contra-lateral limb (control) was performed weekly (1–9 weeks post-injury) in a longitudinal fashion. Acoustic concentration (10*log(mm-3)) was calculated for each ultrasound frame. Houndsfield units were used to calculate HO volume on microCT imaging. Histology was used to confirm the presence of HO...

Abstract 70-weeks-old female C57BL6 senescence marker protein 30 knock out mice exhibited anorexia, lethargy and enlarged abdomen because of an intraperitoneal mass. On necropsy, the mouse revealed a large brown-whitish mass located on mesentery. The also systemic metastasis spread over in various organs. microscopic findings, neoplastic masses were mainly composed round cells characterized by ...

Plasma levels of IL-6 correlate with high blood pressure under many circumstances, and ANG II has been shown to stimulate IL-6 production from various cell types. This study tested the role of IL-6 in mediating the hypertension caused by high-dose ANG II and a high-salt diet. Male C57BL6 and IL-6 knockout (IL-6 KO) mice were implanted with biotelemetry devices and placed in metabolic cages to m...