The DNase test is a simple, economical method that has traditionally been used as a supplemental test to identify pathogenic Staphylococcus. This test also aids in the differentiation of closely-related genera within the Klebsiella-Enterobacter-Serratia division of Enterobacteriaceae and several other pathogens, including screening of C. diphtheriae. Currently DNase activity of microorganisms w...

A modified Burkholder's medium (table 1) autoclaved a t 15 lbs. pressure for 15 minutes after being adjusted to pH 5 was used in 10 ml amounts in broth tubes, or 3 % agar was added for use in petri dishes. Actidione stock solution (10 mg of actidione in 100 ml of water) was sterilized by Seitz filtration, kept in a refrigerator until used, and added aseptically to the medium after autoclaving. ...

Invasive candidiasis (IC) has a high mortality rate of 70%, thus diagnosis should be established without delay. Given its fast result, serological test such as β-d-glucan (BDG) is one alternative modalities. However, it lacks specificity. Candida albicans germ tube antibody (CAGTA) an which sensitivity 76.2% and specificity 80.3%. Manufacturing CAGTA requires provision specific antigen. In this...

An assay procedure to screen for Escherichia coli in foods by using 4-methylumbelliferyl-beta-D-glucuronide (MUG) incorporated into lauryl tryptose (LST) broth was evaluated. The beta-glucuronidase produced by E. coli cleaves the MUG substrate to yield a fluorescent end product. E. coli-negative samples can be identified by lack of fluorescence in LST-MUG within 24 h. MUG was not inhibitory to ...

Tea tree oil, an essential oil from the Australian plant Melaleuca alternifolia, has broad antimicrobial activity and is incorporated into a diverse range of pharmaceutical and cosmetic products (2, 3). Blackwell (1) described a patient with typical signs and symptoms of bacterial vaginosis (BV) who treated herself with tea tree oil vaginal pessaries. After treatment, the patient was symptom fr...

A new antifungal diketopiperazine named haematocin was isolated from the culture broth of Nectria haematococca Berk. et Br. (880701a-1) causing blight disease on ornamental plants, Phalaenopsis spp. and Doritanopsis spp. Its structure was established by spectroscopic methods. Haematocin inhibited the germ-tube elongation and spore-germination of Pyricularia oryzae at the ED50 values of 30 micro...

A spore suspension model and a procedure for recovering ultraviolet (UV)-irradiated spores of Bacillus pumilus were investigated. A most-probable-number tube dilution method using double-strength Trypticase soy broth was found to be superior to the agar plate method for recovering optimal numbers of spores irradiated with sublethal doses of UV energy. Aqueous suspensions of B. pumilus survived ...

Total coliform (TC), fecal coliform (FC), and fecal streptococcus (FS) recoveries in chlorinated secondary sewage effluents were investigated by the membrane filter (MF) and multiple-tube (most probable number, MPN) methods. The LES two-step MF method was found to be comparable to the MPN procedure for determining TC. The TC detection was 1.5 times greater by the LES two-step technique than tha...

In the search for new naturally occurring anti-angiogenic compounds, we found that a culture broth of an unidentified fungal strain B90911 exerted inhibitory activity on capillary-like tube formation of human umbilical vein endothelial cells (HUVEC) in vitro. Four active compounds were isolated by bioassay-guided separation and their structures were identified to be sulochrin (1), methyl asterr...