Soil samples (n=500) collected from different areas of Pakistan were screened for various species of Bacillus by Gram staining, spore staining and by a number of biochemical tests. Seven identified species i.e. B. thuringiensis, B. sphaericus, B. brevis, B. firmus, B. coagulans, B. stearothermophilus and B. megaterium were then administered to the house flies, Musca domestica to ascertain and e...

of B. sphaericru was based on the instar and age of the larvae, i.e., 2nd instar and early to middle-aged 4th instar larvae that fed on B. sphaeri,cuiwere susceptible, while late 4th instar larvae that ceased to feed and pupated prior to ingestion of B. splnerin r survived the bacillus bui succumbed to the effects of Arosurf MSF. Bioassays also indicated that effective dispersal of B. sphaericu...

Entomopathogenic bacteria isolated from Simulium larvae and adults from breeding sites in the states of São Paulo and Rio de Janeiro, Brazil, were identified as 18 strains of Bacillus thuringiensis and one of B. sphaericus. Most of these strains were serotyped according to their flagellar antigens. However, nine of the B. thuringiensis samples, could not be serotyped and were designated as "aut...

In the present study, an efficient mercury-tolerant bacterial strain (RS-5) was isolated from heavy-metalcontaminated industrial effluent. Under shake flask conditions, 97% of the supplemented mercuric chloride was sequestered by the biomass of RS-5 grown in a tryptone soy broth. The sequestered mercuric ions were transformed inside the bacterial cells, as an XRD analysis of the biomass confirm...

The 16S rRNA gene sequences for six strains of Bacillus sphaericus representing five distinct DNA homology groups and one strain of Bacillus fusiformis have been determined. An-oligonucleotide probe based on the area approximately between position 186 and 198 (Escherichia coli numbering) of the 16S rRNA gene of the mosquito pathogen, strain 2362, hybridised to DNA from strains of DNA homology g...

A gram-staining-positive, rod-shaped bacterium, designed strain FJAT-51161T was isolated from farmland soil collected Fujian Province, China. Growth observed at 25–40 °C (optimum 30 °C), pH 7.0–9.0 7.0), and NaCl tolerance in the range of 0–7% (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that belonged to genus Lysinibacillus, had closest relationship wit...

In the course of sporulation, Bacillus sphaericus produces an inclusion body which is toxic to a variety of mosquito larvae. In this review we discuss the general biology of this species and concentrate on the genetics and physiology of toxin production and its processing in the midgut of the larval host. The larvicide of B. sphaericus is unique in that it consists of two proteins of 51 and 42 ...